Journal article
Regional Immune Response to Immunization with Escherichia coli O157:H7-Derived Intimin in Cattle
Clinical and vaccine immunology, Vol.20(4), pp.562-571
04/2013
Handle:
https://hdl.handle.net/2376/107035
PMCID: PMC3623412
PMID: 23408521
Abstract
Escherichia coli
O157:H7 is an enteric pathogen of animals and humans that can result in deadly sequelae. Cattle are asymptomatic carriers and shedders of the bacteria and serve as an important reservoir of human infection.
E. coli
O157:H7 colonizes the gastrointestinal tract, most frequently at the rectoanal junction mucosa in cattle. Vaccination is a potentially highly effective means of decreasing cattle colonization and shedding and thereby decreasing human infections. Currently available vaccines are administered subcutaneously or intramuscularly, and immune responses have been evaluated solely by systemic immunoglobulin responses. This study evaluated local and systemic lymphoproliferative responses in addition to immunoglobulin responses following subcutaneous or mucosal (rectal) immunization with
E. coli
O157:H7 outer membrane protein intimin over three trials. In all three trials, significant local and systemic lymphoproliferative responses (
P
< 0.05) occurred following immunization in the majority of animals, as well as significant immunoglobulin responses (
P
< 0.001) in all animals. Surprisingly, local responses in the mesorectal lymph nodes were very similar between the subcutaneous and mucosal immunization groups. Moreover, the responses in mesorectal lymph nodes appeared targeted rather than generalized, as minimal or no significant responses were observed in the associated prescapular lymph nodes of subcutaneously immunized animals. The results indicate that both subcutaneous and mucosal immunizations are effective methods of inducing immune responses against
E. coli
O157:H7 in cattle.
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Details
- Title
- Regional Immune Response to Immunization with Escherichia coli O157:H7-Derived Intimin in Cattle
- Creators
- Kathryn G Boland - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAAndrea N Hayles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAClaire B Miller - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USATovah Kerr - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAWendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAKevin K Lahmers - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
- Publication Details
- Clinical and vaccine immunology, Vol.20(4), pp.562-571
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900546898801842
- Language
- English
- Resource Type
- Journal article