Journal article
Regulated expression of a calmodulin Isoform alters growth and development in potato
Journal of plant physiology, Vol.149(5), pp.553-558
1996
Handle:
https://hdl.handle.net/2376/107958
PMID: 11540589
Abstract
A transgene approach was taken to study the consequences of altered expression of a calmodulin iso-form on plant growth and development. Eight genomic clones of potato calmodulin (PCM1 to 8) have been isolated and characterized (Takezawa et al., 1995). Among the potato calmodulin isoforms studied, PCM1 differs from the other isoforms because of its unique amino acid substitutions. Transgenic potato plants were produced carrying sense construct of PCM1 fused to the CaMV 35S promoter. Transgenic plants showing a moderate increase in PCM1 mRNA exhibited strong apical dominance, produced elongated tubers, and were taller than the controls. Interestingly, the plants expressing the highest level of PCM1 mRNA did not form underground tubers. Instead, these transgenic plants produced aerial tubers when allowed to grow for longer periods. The expression of different calmodulin isoforms (PCM1, 5, 6, and 8) was studied in transgenic plants. Among the four potato calmodulin isoforms, only the expression of PCM1 mRNA was altered in transgenic plants, while the expression of other isoforms was not significantly altered. Western analysis revealed increased PCM1 protein in transgenic plants, indicating that the expression of both mRNA and protein are altered in transgenic plants. These results suggest that increasing the expression of PCM1 alters growth and development in potato plants.
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Details
- Title
- Regulated expression of a calmodulin Isoform alters growth and development in potato
- Creators
- B.W Poovaiah - Department of Horticulture, Washington State University, Pullman, WA, 99164, USAD Takezawa - Department of Horticulture, Washington State University, Pullman, WA, 99164, USAG An - Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164, USAT.-J Han - Department of Horticulture, Washington State University, Pullman, WA, 99164, USA
- Publication Details
- Journal of plant physiology, Vol.149(5), pp.553-558
- Academic Unit
- Horticulture, Department of
- Publisher
- Elsevier GmbH
- Identifiers
- 99900547450501842
- Language
- English
- Resource Type
- Journal article