Journal article
Regulation and stimulation of photosynthesis of mixotrophically cultured Haematococcus pluvialis by ribose
Algal research (Amsterdam), Vol.39, p.101443
05/2019
Handle:
https://hdl.handle.net/2376/117935
Abstract
The low cell growth rate of Haematococcus pluvialis is a major barrier to achieve low-cost production of the biomass. Our previous study demonstrated that ribose could significantly enhance the growth rate and cell activity of H. pluvialis under mixotrophic conditions. This study showed the photosynthetic rate of the algal cells cultured with ribose was two-fold higher than that of phototrophic after 2-day cultivation. The increased photosynthetic activity of the cells with mixotrophic culture using ribose occurred earlier than that with sodium acetate. Similar photosynthetic fluorescence characteristics was observed for cells grown in phototrophic and ribose-fed culture. Photosystem (PS) I of ribose-fed cells was enlarged relative to PS II after two days. The difference in rubisco activity and subunit gene (rbcL and rbcS) expression however, was not significant between phototrophic and mixotrophic cultivation. The photosynthetic rate of H. pluvialis doubled with ribose mainly due to the high reaction of the Calvin cycle. The results suggested that metabolites derived from ribose could provide additional substrates for the Calvin cycle. This study provides the evidence about unique ribose utilization patterns by H. pluvialis during photosynthesis processes in mixotrophic culture mode. By understanding the mechanism of ribose metabolism in H. pluvialis under mixotrophic cultivation, it offers a promising route for improvement of biomass accumulation.
•Photosynthetic rate of ribose-fed cells of Haematococcus pluvialis is two-fold higher than that of phototrophic culture.•Photosystem (PS) I was enlarged relative to PS II for H. pluvialis cells under ribose-fed mixotrophic culture.•The functional mechanism of ribose for enhancing H. pluvialis growth under mixotrophic conditions was proposed.
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Details
- Title
- Regulation and stimulation of photosynthesis of mixotrophically cultured Haematococcus pluvialis by ribose
- Creators
- Na Pang - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USAYuxiao Xie - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USAHui Min Olivia Oung - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6120, USABalasaheb Vitthal Sonawane - School of Biological Sciences, Washington State University, Pullman, WA 99164-6120, USAXiao Fu - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USAHelmut Kirchhoff - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6120, USAAsaph B Cousins - School of Biological Sciences, Washington State University, Pullman, WA 99164-6120, USAShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA
- Publication Details
- Algal research (Amsterdam), Vol.39, p.101443
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900582333201842
- Language
- English
- Resource Type
- Journal article