Journal article
Regulation of Gene Transcription by the Histone H2A N-Terminal Domain
Molecular and cellular biology, Vol.27(21), pp.7641-7648
11/2007
Handle:
https://hdl.handle.net/2376/114126
PMCID: PMC2169066
PMID: 17724083
Abstract
Histone N-terminal domains play critical roles in regulating chromatin structure and gene transcription. Relatively little is known, however, about the role of the histone H2A N-terminal domain in transcription regulation. We have used DNA microarrays to characterize the changes in genome-wide expression caused by mutations in the N-terminal domain of histone H2A. Our results indicate that the N-terminal domain of histone H2A functions primarily to repress the transcription of a large subset of the
Saccharomyces cerevisiae
genome and that most of the H2A-repressed genes are also repressed by the histone H2B N-terminal domain. Using the histone H2A microarray data, we selected three reporter genes (
BNA1
,
BNA2
, and
GCY1
), which we subsequently used to map regions in the H2A N-terminal domain responsible for this transcriptional repression. These studies revealed that a small subdomain in the H2A N-terminal tail, comprised of residues 16 to 20, is required for the transcriptional repression of these reporter genes. Deletion of either the entire histone H2A N-terminal domain or just this small subdomain imparts sensitivity to UV irradiation. Finally, we show that two residues in this H2A subdomain, serine-17 and arginine-18, are specifically required for the transcriptional repression of the
BNA2
reporter gene.
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Details
- Title
- Regulation of Gene Transcription by the Histone H2A N-Terminal Domain
- Creators
- Michael A Parra - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4660John J Wyrick - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4660
- Publication Details
- Molecular and cellular biology, Vol.27(21), pp.7641-7648
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900547624001842
- Language
- English
- Resource Type
- Journal article