Journal article
Regulation of PKC δ expression by estrogen and rat placental lactogen-1 in luteinized rat ovarian granulosa cells
Molecular and cellular endocrinology, Vol.162(1), pp.181-191
2000
Handle:
https://hdl.handle.net/2376/114359
PMID: 10854711
Abstract
Protein kinase C (PKC) δ is dramatically upregulated in the corpus luteum in the second half of pregnancy in the rat. To gain insight into the hormonal regulation of PKC δ expression, studies were undertaken to analyze the regulation of PKC δ expression in a luteinized rat granulosa cell model. PKC δ protein expression was evaluated in luteinized granulosa cells, isolated from human (h)CG-treated immature female rats 7 h after the injection of an ovulatory dose of hCG and cultured up to 12 days. Cytochrome P450 cholesterol side chain cleavage enzyme expression was observed throughout the culture period, and a majority of the cells expressed steroidogenic acute regulatory protein and responded to rat placental lactogen (rPL)-1 by exhibiting hypertrophy, consistent with maintenance of the luteal phenotype. Both PKC δ protein and mRNA expression increased 3.5–4-fold with time of culture, and PKC δ mRNA expression could be eliminated by treatment of cells with the PKC inhibitor GF109203X.
E
2 caused a specific dose- and time-dependent increase in expression of PKC δ protein of twofold, whereas PKC δ mRNA was unaffected by
E
2 over a 12-day culture period. Treatment of cells with 500 ng/ml rPL-1 for the final 4 days of a 12-day culture in the absence of
E
2 had no effect on PKC δ protein or mRNA expression, while treatment with 500 or 3000 ng/ml rPL-1 in the presence of
E
2 significantly enhanced both PKC δ protein and mRNA expression (up to threefold). These results show that two of the major regulators of luteal function in the second half of pregnancy in the rat,
E
2 and rPL-1, cooperate to regulate PKC δ expression in luteinized granulosa cells.
Metrics
4 Record Views
Details
- Title
- Regulation of PKC δ expression by estrogen and rat placental lactogen-1 in luteinized rat ovarian granulosa cells
- Creators
- Carl A Peters - Department of Cell and Molecular Biology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USARichard E Cutler - Department of Cell and Molecular Biology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USAEvelyn T Maizels - Department of Cell and Molecular Biology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USAMay C Robertson - Department of Physiology, University of Manitoba, Winnipeg, Man., CanadaRobert P Shiu - Department of Physiology, University of Manitoba, Winnipeg, Man., CanadaPhillip Fields - Department of Structural/Cellular Biology, University of South Alabama College of Medicine, Mobile, AL 36688, USAMary Hunzicker-Dunn - Department of Cell and Molecular Biology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USA
- Publication Details
- Molecular and cellular endocrinology, Vol.162(1), pp.181-191
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900548216001842
- Language
- English
- Resource Type
- Journal article