Regulation of gene expression by estrogen and testosterone in the proximal mouse reproductive tract

Elizabeth M Snyder; Christopher L Small; Ying Li; Michael D Griswold

doi:10.1095/biolreprod.109.079053

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Regulation of gene expression by estrogen and testosterone in the proximal mouse reproductive tract

Journal article

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Regulation of gene expression by estrogen and testosterone in the proximal mouse reproductive tract

Elizabeth M Snyder, Christopher L Small, Ying Li and Michael D Griswold

Biology of reproduction, Vol.81(4), pp.707-716

10/2009

DOI: https://doi.org/10.1095/biolreprod.109.079053

Handle:

https://hdl.handle.net/2376/105833

PMID: 19553595

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Abstract

Epididymis - metabolism

Gene Expression - drug effects

Oligonucleotide Array Sequence Analysis

Gene Expression Regulation

Male

Gene Expression Profiling

Orchiectomy

Testosterone - metabolism

Testosterone - administration & dosage

Animals

Mice

Estrogens - metabolism

Estrogens - administration & dosage

The role of estrogen and testosterone in the regulation of gene expression in the proximal reproductive tract is not completely understood. To address this question, mice were treated with testosterone or estradiol, and RNA from the efferent ducts and caput epididymides was processed and hybridized to Affymetrix M430 2.0 microarrays. Analysis of array output identified probe sets in each tissue with altered levels in hormone-treated versus control animals. Hormone treatment efficacy was confirmed by determination of serum hormone levels before and after treatment and by observed changes in transcript levels of previously reported hormone-responsive genes. Tissue-specific hormone sensitivity was observed with 2867 and 3197 probe sets changing significantly in the efferent ducts after estrogen and testosterone treatment, respectively. In the caput epididymidis, 117 and 268 probe sets changed after estrogen and testosterone treatment, respectively, demonstrating a greater response to hormone in the efferent ducts than in the caput epididymidis. Transcripts sharing similar profiles in the intact and hormone-treated animals compared with castrated controls were also identified. Ontology analysis of probe sets revealed that a significant number of hormone-regulated transcripts encode proteins associated with lipid metabolism, transcription, and steroid metabolism in both tissues. Real-time RT-PCR was used to confirm array data and to investigate other potential hormone-responsive regulators of proximal reproductive tract function. The results of this work reveal previously unknown responses to estrogen in the caput epididymidis and to testosterone in the efferent ducts, as well as tissue-specific hormone sensitivity in the proximal reproductive tract.

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Title: Regulation of gene expression by estrogen and testosterone in the proximal mouse reproductive tract
Creators: Elizabeth M Snyder - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington, USA
Christopher L Small
Ying Li
Michael D Griswold
Publication Details: Biology of reproduction, Vol.81(4), pp.707-716
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: U54 HD042454-06 / NICHD NIH HHS U54 HD042454 / NICHD NIH HHS R37 HD010808 / NICHD NIH HHS R01 HD010808 / NICHD NIH HHS R01 HD010808-32 / NICHD NIH HHS
Identifiers: 99900546769601842
Language: English
Resource Type: Journal article