Journal article
Regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase[S]
Journal of lipid research, Vol.54(9), pp.2504-2514
09/2013
Handle:
https://hdl.handle.net/2376/107097
PMCID: PMC3735947
PMID: 23787165
Abstract
Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on fat storage and development in the nematode
Caenorhabditis elegans
, we analyzed the lipid composition and lipid droplet size in the
fat-6;fat-7
desaturase mutants independently and in combination with mutants disrupted in conserved lipid metabolic pathways.
C. elegans
with impaired SCD activity displayed both reduced fat stores and decreased lipid droplet size. Mutants in the
daf-2
(insulin-like growth factor receptor),
rsks-1
(homolog of p70S6kinase, an effector of the target of rapamycin signaling pathway), and
daf-7
(transforming growth factor β) displayed high fat stores, the opposite of the low fat observed in the
fat-6;fat-7
desaturase mutants. The metabolic mutants in combination with
fat-6;fat-7
displayed low fat stores, with the exception of the
daf-2;fat-6;fat-7
triple mutants, which had increased de novo fatty acid synthesis and wild-type levels of fat stores. Notably, SCD activity is required for the formation of large-sized lipid droplets in all mutant backgrounds, as well as for normal ratios of phosphatidylcholine (PC) to phosphatidylethanolamine (PE). These studies reveal previously uncharacterized roles for SCD in the regulation of lipid droplet size and membrane phospholipid composition.
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Details
- Title
- Regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase[S]
- Creators
- Xun Shi - School of Molecular BiosciencesJuan Li - Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, theXiaoju Zou - School of Molecular BiosciencesJoel Greggain - School of Molecular BiosciencesSteven V Rødkær - Department of Biochemistry and Molecular BiologyNils J Færgeman - Department of Biochemistry and Molecular BiologyBin Liang - School of Molecular BiosciencesJennifer L Watts - School of Molecular Biosciences
- Publication Details
- Journal of lipid research, Vol.54(9), pp.2504-2514
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Identifiers
- 99900546808701842
- Language
- English
- Resource Type
- Journal article