Journal article
Regulation of neural specification from human embryonic stem cells by BMP and FGF
Stem cells (Dayton, Ohio), Vol.27(8), pp.1741-1749
08/2009
Handle:
https://hdl.handle.net/2376/108218
PMCID: PMC2789116
PMID: 19544434
Abstract
Inhibition of bone morphogenetic protein (BMP) signaling is required for vertebrate neural induction, and fibroblast growth factors (FGFs) may affect neural induction through phosphorylation at the linker region of Smad1, thus regulating BMP signaling. Here we show that human embryonic stem cells efficiently convert to neuroepithelial cells in the absence of BMP antagonists, or even when exposed to high concentrations of exogenous BMP4. Molecular and functional analyses revealed multiple levels of endogenous BMP signaling inhibition that may account for the efficient neural differentiation. Blocking FGF signaling inhibited neural induction, but did not alter the phosphorylation of the linker region of Smad1, suggesting that FGF enhances human neural specification independently of BMP signaling.
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Details
- Title
- Regulation of neural specification from human embryonic stem cells by BMP and FGF
- Creators
- Timothy M LaVaute - Neuroscience Training Program, School of Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USAYoung Dong YooMatthew T PankratzJason P WeickJason R GerstnerSu-Chun Zhang
- Publication Details
- Stem cells (Dayton, Ohio), Vol.27(8), pp.1741-1749
- Academic Unit
- Biomedical Sciences, Department of
- Publisher
- United States
- Grant note
- R01 NS045926-01 / NINDS NIH HHS P30 HD003352-40 / NICHD NIH HHS R01 NS045926-03 / NINDS NIH HHS R01 NS045926-02S1 / NINDS NIH HHS T32 GM007507 / NIGMS NIH HHS P30 HD003352 / NICHD NIH HHS R01 NS045926 / NINDS NIH HHS R01 NS045926-05 / NINDS NIH HHS R01 NS045926-04 / NINDS NIH HHS R01 NS045926-02 / NINDS NIH HHS P30 HD03352 / NICHD NIH HHS
- Identifiers
- 99900547253401842
- Language
- English
- Resource Type
- Journal article