Regulation of protein tyrosine phosphatase 4a1, B-cell translocation gene 2, nuclear receptor subfamily 4a1 and diacylglycerol O-acyltransferase 1 by follicle stimulating hormone in the rat ovary

Jonathan Schmidt; Jeanene de Avila; Derek McLean

doi:10.1071/RD05167

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Regulation of protein tyrosine phosphatase 4a1, B-cell translocation gene 2, nuclear receptor subfamily 4a1 and diacylglycerol O-acyltransferase 1 by follicle stimulating hormone in the rat ovary

Journal article

Peer reviewed

Regulation of protein tyrosine phosphatase 4a1, B-cell translocation gene 2, nuclear receptor subfamily 4a1 and diacylglycerol O-acyltransferase 1 by follicle stimulating hormone in the rat ovary

Jonathan Schmidt, Jeanene de Avila and Derek McLean

Reproduction fertility and development, Vol.18(7), pp.757-765

2006

DOI: https://doi.org/10.1071/RD05167

Handle:

https://hdl.handle.net/2376/117479

PMID: 17032584

Abstract

Immunohistochemistry

Receptors, Steroid - metabolism

Nuclear Receptor Subfamily 4, Group A, Member 1

Protein Tyrosine Phosphatases - metabolism

Neoplasm Proteins - metabolism

DNA-Binding Proteins - metabolism

Immediate-Early Proteins - metabolism

Protein Tyrosine Phosphatases - genetics

Female

Ovary - drug effects

Neoplasm Proteins - genetics

Ovary - metabolism

Follicle Stimulating Hormone - metabolism

Gene Expression Regulation

Rats

Receptors, Cytoplasmic and Nuclear - genetics

Transcription Factors - genetics

DNA-Binding Proteins - genetics

Rats, Sprague-Dawley

Transcription Factors - metabolism

Animals

Immediate-Early Proteins - genetics

Ovary - enzymology

Diacylglycerol O-Acyltransferase - genetics

Receptors, Steroid - genetics

Diacylglycerol O-Acyltransferase - metabolism

Receptors, Cytoplasmic and Nuclear - metabolism

Ovarian response to follicle stimulating hormone (FSH) and luteinising hormone (LH) leads to the formation of a mature follicle that is eventually ovulated. FSH and LH are essential for this process because they direct changes in somatic cells associated with folliculogenesis by regulating the expression of multiple genes. We hypothesised that genes induced by FSH in rat Sertoli cells would also show hormonal regulation during rat folliculogenesis. The objective of this study was to determine the expression patterns of diacylglycerol O-acyltransferase 1 (Dgat1), nuclear receptor subfamily 4a1 (Nr4a1), an anti-proliferative gene (Btg2) and a protein tyrosine phosphatase (Ptp4a1) in the ovaries of pregnant mare serum gonadotrophin (PMSG)-treated and human chorionic gonadotrophin (hCG)-treated rats. Expression of Dgat1, Nr4a1 and Ptp4a1 was induced in ovaries 4 h post PMSG treatment. When rats were treated with hCG, Dgat1, Nr4a1 and Ptp4a1 expression was induced by 12 h. Expression of Nr4a1 protein increases 12-24 h after induction of gene expression. Nr4a1 protein was observed in the granulosa, theca and luteal cells post PMSG and hCG treatment. These findings should increase our knowledge of mechanisms regulating folliculogenesis and luteinisation and demonstrate the diverse proteins that are important in ovarian function.

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Title: Regulation of protein tyrosine phosphatase 4a1, B-cell translocation gene 2, nuclear receptor subfamily 4a1 and diacylglycerol O-acyltransferase 1 by follicle stimulating hormone in the rat ovary
Creators: Jonathan Schmidt - Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA
Jeanene de Avila
Derek McLean
Publication Details: Reproduction fertility and development, Vol.18(7), pp.757-765
Academic Unit: Center for Reproductive Biology
Publisher: Australia
Identifiers: 99900547842701842
Language: English
Resource Type: Journal article

Regulation of protein tyrosine phosphatase 4a1, B-cell translocation gene 2, nuclear receptor subfamily 4a1 and diacylglycerol O-acyltransferase 1 by follicle stimulating hormone in the rat ovary

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