Journal article
Regulation of the Docosapentaenoic Acid/Docosahexaenoic Acid Ratio (DPA/DHA Ratio) in Schizochytrium limacinum B4D1
Applied biochemistry and biotechnology, Vol.182(1), pp.67-81
05/2017
Handle:
https://hdl.handle.net/2376/117328
PMID: 27832512
Abstract
Docosapentaenoic acid/docosahexaenoic acid ratio (DPA/DHA ratio) in Schizochytrium was relatively stable. But ideally the ratio of DPA/DHA will vary according to the desired end use. This study reports several ways of modulating the DPA/DHA ratio. Incubation times changed the DPA/DHA ratio, and changes in this ratio were associated with the variations in the saturated fatty acid (SFAs) content. Propionic acid sharply increased the SFAs content in lipids, dramatically decreased the even-chain SFAs content, and reduced the DPA/DHA ratio. Pentanoic acid (C5:0) and heptanoic acid (C7:0) had similar effects as propionic acid, whereas butyric acid (C4:0), hexanoic acid (C6:0), and octanoic acid (C8:0) did not change the fatty acid profile and the DPA/DHA ratio. Transcription analyses show that β-oxidation might be responsible for this phenomenon. Iodoacetamide upregulated polyunsaturated fatty acid (PUFA) synthase genes, reduced the DHA content, and improved the DPA content, causing the DPA/DHA ratio to increase. These results present new insights into the regulation of the DPA/DHA ratio.
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Details
- Title
- Regulation of the Docosapentaenoic Acid/Docosahexaenoic Acid Ratio (DPA/DHA Ratio) in Schizochytrium limacinum B4D1
- Creators
- Ke Zhang - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaHuidong Li - Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin, 300100, ChinaWuxi Chen - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaMinli Zhao - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaHaiyang Cui - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaQingsong Min - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaHaijun Wang - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaShulin Chen - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, ChinaDemao Li - Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. li_dm@tib.cas.cn
- Publication Details
- Applied biochemistry and biotechnology, Vol.182(1), pp.67-81
- Academic Unit
- Biological Systems Engineering, Department of
- Identifiers
- 99900582322801842
- Language
- English
- Resource Type
- Journal article