Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes

Tracy M Clement; Matthew D Anway; Mehmet Uzumcu; Michael K Skinner

doi:10.1530/REP-06-0341

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Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes

Journal article

Open access

Peer reviewed

Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes

Tracy M Clement, Matthew D Anway, Mehmet Uzumcu and Michael K Skinner

Reproduction (Cambridge, England), Vol.134(3), pp.455-472

09/2007

DOI: https://doi.org/10.1530/REP-06-0341

Handle:

https://hdl.handle.net/2376/110097

PMCID: PMC8260008

PMID: 17709564

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Abstract

Morphogenesis - genetics

Oligonucleotide Array Sequence Analysis

Ovary - embryology

Embryonic Development - genetics

Gene Expression Profiling

Sex Differentiation

Gene Regulatory Networks

Rats, Sprague-Dawley

Transcription, Genetic - physiology

Sex Determination Processes

Testis - embryology

Gene Expression Regulation, Developmental

Organ Culture Techniques

Computational Biology

Gene expression profiles during sex determination and gonadal differentiation were investigated to identify new potential regulatory factors. Embryonic day 13 (E13), E14, and E16 rat testes and ovaries were used for microarray analysis, as well as E13 testis organ cultures that undergo testis morphogenesis and develop seminiferous cords in vitro. A list of 109 genes resulted from a selective analysis for genes present in male gonadal development and with a 1.5-fold change in expression between E13 and E16. Characterization of these 109 genes potentially important for testis development revealed that cytoskeletal-associated proteins, extracellular matrix factors, and signaling factors were highly represented. Throughout the developmental period (E13-E16), sex-enriched transcripts were more prevalent in the male with 34 of the 109 genes having testis-enriched expression during sex determination. In ovaries, the total number of transcripts with a 1.5-fold change in expression between E13 and E16 was similar to the testis, but none of those genes were both ovary enriched and regulated during the developmental period. Genes conserved in sex determination were identified by comparing changing transcripts in the rat analysis herein, to transcripts altered in previously published mouse studies of gonadal sex determination. A comparison of changing mouse and rat transcripts identified 43 genes with species conservation in sex determination and testis development. Profiles of gene expression during E13-E16 rat testis and ovary development are presented and candidate genes for involvement in sex determination and testis differentiation are identified. Analysis of cellular pathways did not reveal any specific pathways involving multiple candidate genes. However, the genes and gene network identified influence numerous cellular processes with cellular differentiation, proliferation, focal contact, RNA localization, and development being predominant.

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Title: Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes
Creators: Tracy M Clement - School of Molecular Biosciences, Center for Reproductive Biology, Washington State University, Pullman, Washington 99164, USA
Matthew D Anway
Mehmet Uzumcu
Michael K Skinner
Publication Details: Reproduction (Cambridge, England), Vol.134(3), pp.455-472
Academic Unit: Biological Sciences, School of
Publisher: England
Identifiers: 99900547354201842
Language: English
Resource Type: Journal article