Relatedness of structures of a major immunogen in Trichomonas vaginalis isolates

J F Alderete; K A Neale

doi:10.1128/IAI.57.6.1849-1853.1989

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Relatedness of structures of a major immunogen in Trichomonas vaginalis isolates

Journal article

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Relatedness of structures of a major immunogen in Trichomonas vaginalis isolates

J F Alderete and K A Neale

Infection and immunity, Vol.57(6), pp.1849-1853

06/1989

DOI: https://doi.org/10.1128/IAI.57.6.1849-1853.1989

Handle:

https://hdl.handle.net/2376/104256

PMCID: PMC313366

PMID: 2785964

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https://doi.org/10.1128/IAI.57.6.1849-1853.1989View

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Abstract

Antigens, Protozoan - immunology

Trichomonas vaginalis - isolation & purification

Antigens, Protozoan - biosynthesis

Antibodies, Monoclonal

Precipitin Tests

Autolysis

Hydrolysis

Phenotype

Trichomonas vaginalis - immunology

Animals

Trichomonas vaginalis - physiology

Antigens, Protozoan - isolation & purification

Hydrogen-Ion Concentration

Solubilization of live Trichomonas vaginalis organisms with detergent caused the release of cysteine proteinases in the detergent extract which were inhibitable with N-alpha-p-tosyl-L-lysine chloromethyl ketone. The detergent extracts of all isolates tested possessed similar cysteine proteinase activities. These parasite proteinases rapidly degraded a prominent immunogen whose surface disposition undergoes phenotypic variation in some isolates. The relatedness of the forms of this immunogen among all isolates tested was confirmed by identical immunoblot patterns of autolysed immunogen, and data suggest the presence of repeating units or at least equidistant sites for proteinase cleavage within the immunogen molecule.

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Title: Relatedness of structures of a major immunogen in Trichomonas vaginalis isolates
Creators: J F Alderete - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758
K A Neale
Publication Details: Infection and immunity, Vol.57(6), pp.1849-1853
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: AI-22380 / NIAID NIH HHS K04-AI-00584 / NIAID NIH HHS
Identifiers: 99900546558301842
Language: English
Resource Type: Journal article