Journal article
Reorganization of Acyl Flux through the Lipid Metabolic Network in Oil-Accumulating Tobacco Leaves
Plant physiology (Bethesda), Vol.182(2), pp.739-755
02/01/2020
PMCID: PMC6997700
PMID: 31792147
Abstract
The triacylglycerols (TAGs; i.e. oils) that accumulate in plants represent the most energy-dense form of biological carbon storage, and are used for food, fuels, and chemicals. The increasing human population and decreasing amount of arable land have amplified the need to produce plant oil more efficiently. Engineering plants to accumulate oils in vegetative tissues is a novel strategy, because most plants only accumulate large amounts of lipids in the seeds. Recently, tobacco (
) leaves were engineered to accumulate oil at 15% of dry weight due to a push (increased fatty acid synthesis)-and-pull (increased final step of TAG biosynthesis) engineering strategy. However, to accumulate both TAG and essential membrane lipids, fatty acid flux through nonengineered reactions of the endogenous metabolic network must also adapt, which is not evident from total oil analysis. To increase our understanding of endogenous leaf lipid metabolism and its ability to adapt to metabolic engineering, we utilized a series of in vitro and in vivo experiments to characterize the path of acyl flux in wild-type and transgenic oil-accumulating tobacco leaves. Acyl flux around the phosphatidylcholine acyl editing cycle was the largest acyl flux reaction in wild-type and engineered tobacco leaves. In oil-accumulating leaves, acyl flux into the eukaryotic pathway of glycerolipid assembly was enhanced at the expense of the prokaryotic pathway. However, a direct Kennedy pathway of TAG biosynthesis was not detected, as acyl flux through phosphatidylcholine preceded the incorporation into TAG. These results provide insight into the plasticity and control of acyl lipid metabolism in leaves.
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Details
- Title
- Reorganization of Acyl Flux through the Lipid Metabolic Network in Oil-Accumulating Tobacco Leaves
- Creators
- Xue-Rong Zhou - Agriculture and FoodSajina Bhandari - Washington State UniversityBrandon S Johnson - Washington State University, Pullman, Washington 99164-6340Hari Kiran Kotapati - Washington State University, Pullman, Washington 99164-6340Doug K Allen - United States Department of Agriculture-Agricultural Research Service, Donald Danforth Plant Science Center, St. Louis, Missouri 63132Thomas Vanhercke - Agriculture and FoodPhilip D Bates - Washington State University, Biological Chemistry, Institute of
- Publication Details
- Plant physiology (Bethesda), Vol.182(2), pp.739-755
- Academic Unit
- Biological Chemistry, Institute of
- Identifiers
- 99900970440801842
- Language
- English
- Resource Type
- Journal article