Journal article
Repair of DNA Double-Strand Breaks following UV Damage in Three Sulfolobus solfataricus Strains
Journal of bacteriology, Vol.192(19), pp.4954-4962
10/2010
Handle:
https://hdl.handle.net/2376/108401
PMCID: PMC2944543
PMID: 20675475
Abstract
DNA damage repair mechanisms have been most thoroughly explored in the eubacterial and eukaryotic branches of life. The methods by which members of the archaeal branch repair DNA are significantly less well understood but have been gaining increasing attention. In particular, the approaches employed by hyperthermophilic archaea have been a general source of interest, since these organisms thrive under conditions that likely lead to constant chromosomal damage. In this work we have characterized the responses of three
Sulfolobus solfataricus
strains to UV-C irradiation, which often results in double-strand break formation. We examined
S. solfataricus
strain P2 obtained from two different sources and
S. solfataricus
strain 98/2, a popular strain for site-directed mutation by homologous recombination. Cellular recovery, as determined by survival curves and the ability to return to growth after irradiation, was found to be strain specific and differed depending on the dose applied. Chromosomal damage was directly visualized using pulsed-field gel electrophoresis and demonstrated repair rate variations among the strains following UV-C irradiation-induced double-strand breaks. Several genes involved in double-strand break repair were found to be significantly upregulated after UV-C irradiation. Transcript abundance levels and temporal expression patterns for double-strand break repair genes were also distinct for each strain, indicating that these
Sulfolobus solfataricus
strains have differential responses to UV-C-induced DNA double-strand break damage.
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Details
- Title
- Repair of DNA Double-Strand Breaks following UV Damage in Three Sulfolobus solfataricus Strains
- Creators
- Michael L Rolfsmeier - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Marian F Laughery - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Cynthia A Haseltine - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
- Publication Details
- Journal of bacteriology, Vol.192(19), pp.4954-4962
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900547041601842
- Language
- English
- Resource Type
- Journal article