Journal article
Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop
Veterinary immunology and immunopathology, Vol.60(3), pp.291-303
1998
Handle:
https://hdl.handle.net/2376/114335
PMID: 9589568
Abstract
Based on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, #090; UCP1H12-2, #139; and PG164A, #051) bind exclusively to CD8
+ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 33 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8
+ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8
low and CD8
high cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8
low and CD8
high lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4
−/CD8
high lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, #094; and SwNL554.1, #009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8
+ lymphocyte subsets in health and disease.
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Details
- Title
- Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop
- Creators
- F.A Zuckermann - University of Illinois, Dept. of Veterinary Pathobiology, 2001 So. Lincoln Ave., Urbana, IL 61801, USAM.D Pescovitz - Indiana University, Dept. of Surgery and Microbiology/Immunology, Indianapolis, IN, USAB Aasted - Royal Veterinary University, Frederikberg C, DenmarkJ Dominguez - Departamento de Sanidad Animal, INIA, Madrid, SpainI Trebichavsky - Institute Microbiology, Prague, Czech RepublicB Novikov - All-Russian Research Institute of Veterinary Virology and Microbiology, Vlasimizskaya obl., Russian FederationI Valpotic - Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, CroatiaJ Nielsen - State Veterinary Institute for Virus Research, Kalvehave, DenmarkS Arn - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAD.H Sachs - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAJ.K Lunney - USDA, ARS, Immunology and Disease Resistance Laboratory, Beltsville, MD, USAP Boyd - USDA, ARS, Immunology and Disease Resistance Laboratory, Beltsville, MD, USAJ Walker - Center for Animal Biotechnology, University of Melbourne, Victoria, AustraliaR Lee - Center for Animal Biotechnology, University of Melbourne, Victoria, AustraliaW.C Davis - Washington State University, Pullman, WA, USAI.R Barbosa - Washington State University, Pullman, WA, USAA Saalmüller - Federal Research Center for Virus Diseases of Animals, Tübingen, Germany
- Publication Details
- Veterinary immunology and immunopathology, Vol.60(3), pp.291-303
- Academic Unit
- Medical Education and Clinical Science, Department of; Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548012001842
- Language
- English
- Resource Type
- Journal article