Repression of Sulfate Assimilation Is an Adaptive Response of Yeast to the Oxidative Stress of Zinc Deficiency

Chang-Yi Wu; Sanja Roje; Francisco J Sandoval; Amanda J Bird; Dennis R Winge; David J Eide

doi:10.1074/jbc.M109.042036

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Repression of Sulfate Assimilation Is an Adaptive Response of Yeast to the Oxidative Stress of Zinc Deficiency

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Repression of Sulfate Assimilation Is an Adaptive Response of Yeast to the Oxidative Stress of Zinc Deficiency

Chang-Yi Wu, Sanja Roje, Francisco J Sandoval, Amanda J Bird, Dennis R Winge and David J Eide

The Journal of biological chemistry, Vol.284(40), pp.27544-27556

10/02/2009

DOI: https://doi.org/10.1074/jbc.M109.042036

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https://hdl.handle.net/2376/109021

PMCID: PMC2785683

PMID: 19656949

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Abstract

Metabolism and Bioenergetics

The Zap1 transcription factor is a central player in the response of yeast to changes in zinc status. Previous studies identified over 80 genes activated by Zap1 in zinc-limited cells. In this report, we identified 36 genes repressed in a zinc- and Zap1-responsive manner. As a result, we have identified a new mechanism of Zap1-mediated gene repression whereby transcription of the MET3 , MET14 , and MET16 genes is repressed in zinc-limited cells. These genes encode the first three enzymes of the sulfate assimilation pathway. We found that MET30 , encoding a component of the SCF Met30 ubiquitin ligase, is a direct Zap1 target gene. MET30 expression is increased in zinc-limited cells, and this leads to degradation of Met4, a transcription factor responsible for MET3 , MET14 , and MET16 expression. Thus, Zap1 is responsible for a decrease in sulfate assimilation in zinc-limited cells. We further show that cells that are unable to down-regulate sulfate assimilation under zinc deficiency experience increased oxidative stress. This increased oxidative stress is associated with an increase in the NADP + /NADPH ratio and may result from a decrease in NADPH-dependent antioxidant activities. These studies have led to new insights into how cells adapt to nutrient-limiting growth conditions.

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Title: Repression of Sulfate Assimilation Is an Adaptive Response of Yeast to the Oxidative Stress of Zinc Deficiency
Creators: Chang-Yi Wu - From the
Sanja Roje - the
Francisco J Sandoval - the
Amanda J Bird - the
Dennis R Winge - the
David J Eide - From the
Publication Details: The Journal of biological chemistry, Vol.284(40), pp.27544-27556
Academic Unit: Biological Chemistry, Institute of
Publisher: American Society for Biochemistry and Molecular Biology; 9650 Rockville Pike, Bethesda, MD 20814, U.S.A
Grant note: GM56285 / National Institutes of Health
Identifiers: 99900547401201842
Language: English
Resource Type: Journal article