Journal article
Resident Enteric Bacteria Are Necessary for Development of Spontaneous Colitis and Immune System Activation in Interleukin-10-Deficient Mice
Infection and immunity, Vol.66(11), pp.5224-5231
11/1998
Handle:
https://hdl.handle.net/2376/110748
PMCID: PMC108652
PMID: 9784526
Abstract
Mice with targeted deletion of the gene for interleukin-10 (IL-10) spontaneously develop enterocolitis when maintained in conventional conditions but develop only colitis when kept in specific-pathogen-free (SPF) environments. This study tested the hypothesis that enteric bacteria are necessary for the development of spontaneous colitis and immune system activation in IL-10-deficient mice. IL-10-deficient mice were maintained in either SPF conditions or germfree conditions or were populated with bacteria known to cause colitis in other rodent models. IL-10-deficient mice kept in SPF conditions developed colitis in all segments of the colon (cecum and proximal and distal colon). These mice exhibited immune system activation as evidenced by increased expression of CD44 on CD4
+
T cells; increased mesenteric lymph node cell numbers; and increased production of immunoglobulin A (IgA), IgG1, and IL-12 p40 from colon fragment cultures. Mice populated with bacterial strains, including
Bacteroides vulgatus
, known to induce colitis in other rodent models had minimal colitis. Germfree IL-10-deficient mice had no evidence of colitis or immune system activation. We conclude therefore that resident enteric bacteria are necessary for the development of spontaneous colitis and immune system activation in IL-10-deficient mice.
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Details
- Title
- Resident Enteric Bacteria Are Necessary for Development of Spontaneous Colitis and Immune System Activation in Interleukin-10-Deficient Mice
- Creators
- Rance K Sellon - Department of Companion Animal and Special SpeciesSusan Tonkonogy - Department of Companion Animal and Special SpeciesMichael Schultz - Department of Companion Animal and Special SpeciesLevinus A Dieleman - Department of Companion Animal and Special SpeciesWetonia Grenther - Department of Companion Animal and Special SpeciesEd Balish - Department of Companion Animal and Special SpeciesDonna M Rennick - Department of Companion Animal and Special SpeciesR. Balfour Sartor - Department of Companion Animal and Special Species
- Publication Details
- Infection and immunity, Vol.66(11), pp.5224-5231
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547303501842
- Language
- English
- Resource Type
- Journal article