Journal article
Resistance Gene Analog Polymorphism (RGAP) Markers Co-Localize with Disease Resistance Genes and QTL in Common Bean
Molecular breeding, Vol.17(2), pp.127-135
02/2006
Handle:
https://hdl.handle.net/2376/113455
Abstract
Resistance (R) genes containing nucleotide-binding site (NBS)-leucine rich repeats (LRR) are the most prevalent types of R gene in plants. The objective of this study was to develop PCR-based R-gene analog polymorphism (RGAP) markers for common bean (Phaseolus vulgaris L). Twenty degenerate primers were designed from the conserved kinase-1a (GVGKTT) and hydrophobic domains (GLPLAL) of known NBS-LRR type R-genes and from EST databases. Sixty-six of the 100 primer combinations tested yielded polymorphism. Thirty-two RGAP markers were mapped in the BAT 93/Jalo EEP558 core mapping population for common bean. The markers mapped to 10 of 11 linkage groups with a strong tendency for clustering. In addition, the RGAP markers co-located, on six linkage groups, with 15 resistance gene analogs (RGAs) that were previously mapped in other populations of common bean. The distance between the priming sites in NBS-LRR type R-genes is around 500 bp. Of the 32 RGAP markers, 19 had sizes larger and 13 less than 500 bp. RGAP markers mapped close to known R-genes on B11, and to QTLs for resistance on B1, B2, B6, B7, B8, B10, and B11. RGAP appears to provide a useful marker technique for tagging and mapping R-genes in segregating common bean populations, discovery of candidate genes underlying resistance QTL, and future cloning of R-genes in common bean.
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Details
- Title
- Resistance Gene Analog Polymorphism (RGAP) Markers Co-Localize with Disease Resistance Genes and QTL in Common Bean
- Creators
- Nedim Mutlu - Department of Agronomy and Horticulture University of Nebraska Lincoln NE 68683 USAPhillip Miklas - USDA-ARS Vegetable and Forage Crop Research Unit 24106 North Bunn Road Prosser WA 99350 USADermot Coyne - Department of Agronomy and Horticulture University of Nebraska Lincoln NE 68683 USA
- Publication Details
- Molecular breeding, Vol.17(2), pp.127-135
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900547973501842
- Language
- English
- Resource Type
- Journal article