Journal article
Restriction of major surface protein 2 (MSP2) variants during tick transmission of the ehrlichia Anaplasma marginale
Proceedings of the National Academy of Sciences - PNAS, Vol.96(6), pp.3171-3176
03/16/1999
Handle:
https://hdl.handle.net/2376/100460
PMCID: PMC15914
PMID: 10077656
Abstract
Anaplasma marginale
is an ehrlichial pathogen of cattle that establishes lifelong persistent infection. Persistence is characterized by rickettsemic cycles in which new
A. marginale
variant types, defined by the sequence of the expressed
msp2
transcripts, emerge. The polymorphic
msp2
transcripts encode structurally distinct MSP2 proteins and result in an antigenically diverse and continually changing
A. marginale
population within the blood. In this manuscript, we used sequence analysis of
msp2
transcripts to show that a restricted repertoire of variant types, designated SGV1 and SGV2, is expressed within the tick salivary gland. The same SGV1 and SGV2 variant types were expressed in ticks regardless of the variant types expressed in the blood of infected cattle at the time of acquisition feeding by the ticks. Importantly, subsequent tick transmission to susceptible cattle resulted in acute rickettsemia composed of organisms expressing only the same SGV1 and SGV2 variant types. This indicates that the
msp2
expressed by organisms within the tick salivary gland predicts the variant type responsible for acute rickettsemia and disease. This restriction of transmitted
A. marginale
variant types, in contrast to the marked diversity within persistently infected cattle, supports development of MSP2 vaccines to prevent acute rickettsemia in tick-transmitted infections.
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Details
- Title
- Restriction of major surface protein 2 (MSP2) variants during tick transmission of the ehrlichia Anaplasma marginale
- Creators
- Fred R Rurangirwa - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040; andDavid Stiller - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040; andDorothy M French - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040; andGuy H Palmer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040; and
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.96(6), pp.3171-3176
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- The National Academy of Sciences
- Identifiers
- 99900546784601842
- Language
- English
- Resource Type
- Journal article