Journal article
Retinoic acid activates two pathways required for meiosis in mice
PLoS genetics, Vol.10(8), pp.e1004541-e1004541
08/2014
Handle:
https://hdl.handle.net/2376/104241
PMCID: PMC4125102
PMID: 25102060
Abstract
In all sexually reproducing organisms, cells of the germ line must transition from mitosis to meiosis. In mice, retinoic acid (RA), the extrinsic signal for meiotic initiation, activates transcription of Stra8, which is required for meiotic DNA replication and the subsequent processes of meiotic prophase. Here we report that RA also activates transcription of Rec8, which encodes a component of the cohesin complex that accumulates during meiotic S phase, and which is essential for chromosome synapsis and segregation. This RA induction of Rec8 occurs in parallel with the induction of Stra8, and independently of Stra8 function, and it is conserved between the sexes. Further, RA induction of Rec8, like that of Stra8, requires the germ-cell-intrinsic competence factor Dazl. Our findings strengthen the importance of RA and Dazl in the meiotic transition, provide important details about the Stra8 pathway, and open avenues to investigate early meiosis through analysis of Rec8 induction and function.
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Details
- Title
- Retinoic acid activates two pathways required for meiosis in mice
- Creators
- Jana Koubova - Whitehead Institute, Cambridge, Massachusetts, United States of America; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of AmericaYueh-Chiang Hu - Whitehead Institute, Cambridge, Massachusetts, United States of AmericaTanmoy Bhattacharyya - Whitehead Institute, Cambridge, Massachusetts, United States of AmericaY Q Shirleen Soh - Whitehead Institute, Cambridge, Massachusetts, United States of America; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of AmericaMark E Gill - Whitehead Institute, Cambridge, Massachusetts, United States of AmericaMary L Goodheart - Whitehead Institute, Cambridge, Massachusetts, United States of America; Howard Hughes Medical Institute, Whitehead Institute, Cambridge, Massachusetts, United States of AmericaCathryn A Hogarth - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington, United States of AmericaMichael D Griswold - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington, United States of AmericaDavid C Page - Whitehead Institute, Cambridge, Massachusetts, United States of America; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; Howard Hughes Medical Institute, Whitehead Institute, Cambridge, Massachusetts, United States of America
- Publication Details
- PLoS genetics, Vol.10(8), pp.e1004541-e1004541
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- Howard Hughes Medical Institute U54 HD042454 / NICHD NIH HHS R01 HD010808 / NICHD NIH HHS
- Identifiers
- 99900546871301842
- Language
- English
- Resource Type
- Journal article