Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for controlled release of bovine serum albumin

Sudip Dasgupta; Amit Bandyopadhyay; Susmita Bose

doi:10.1016/j.actbio.2009.04.031

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Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for controlled release of bovine serum albumin

Journal article

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Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for controlled release of bovine serum albumin

Sudip Dasgupta, Amit Bandyopadhyay and Susmita Bose

Acta biomaterialia, Vol.5(8), pp.3112-3121

2009

DOI: https://doi.org/10.1016/j.actbio.2009.04.031

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https://hdl.handle.net/2376/111089

PMCID: PMC2825089

PMID: 19435617

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https://doi.org/10.1016/j.actbio.2009.04.031View

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Abstract

Tricalcium phosphate (TCP)

Reverse micelle

Calcium-deficient hydroxyapatite (CDHA) phase

Protein

Controlled release

Calcium phosphate (CaP) nanoparticles with a calcium to phosphorus (Ca:P) molar ratio of 1.5:1 were synthesized using reverse microemulsion. Ca(NO 3) 2·4H 2O and H 3PO 4 were used as the aqueous phase, cyclohexane as the organic phase and poly(oxyethylene) 12 nonylphenol ether (NP-12) as the surfactant. Depending on the calcination temperature between 600 and 800 °C, CaP nanoparticle showed different phases of calcium-deficient hydroxyapatite (CDHA) and β-tricalcium phosphate (β-TCP), particle size between 48 and 69 nm, and a BET specific average surface area between 73 and 57 m 2 g −1. Bovine serum albumin (BSA) was used as a model protein to study loading and release behavior. The adsorptive property of BSA was investigated by the change in BET surface area of these nanoparticles and the pH of the suspension. At pH 7.5, the maximum amount of BSA was adsorbed onto CaP nanoparticle. The release kinetics of BSA showed a gradual time-dependent increase in pH 4.0 and 6.0 buffer solutions. However, the amount of protein released was significantly smaller at pH 7.2. The BSA release rate also varied depending on the presence of different phases of CaPs in the system, β-TCP or CDHA. These results suggest that the BSA protein release rate can be controlled by changing the particle size, surface area and phase composition of the CaP nanocarriers.

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Title: Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for controlled release of bovine serum albumin
Creators: Sudip Dasgupta
Amit Bandyopadhyay
Susmita Bose
Publication Details: Acta biomaterialia, Vol.5(8), pp.3112-3121
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: Elsevier Ltd
Identifiers: 99900547364801842
Language: English
Resource Type: Journal article