Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell–cell fusion

Rachel M Schowalter; Mark A Wurth; Hector C Aguilar; Benhur Lee; Carole L Moncman; Richard O McCann; Rebecca Ellis Dutch

doi:10.1016/j.virol.2006.01.033

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Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell–cell fusion

Journal article

Open access

Peer reviewed

Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell–cell fusion

Rachel M Schowalter, Mark A Wurth, Hector C Aguilar, Benhur Lee, Carole L Moncman, Richard O McCann and Rebecca Ellis Dutch

Virology (New York, N.Y.), Vol.350(2), pp.323-334

2006

DOI: https://doi.org/10.1016/j.virol.2006.01.033

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https://hdl.handle.net/2376/104576

PMID: 16500690

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Abstract

Membrane fusion

Actin

Rho GTPase

Paramyxovirus

Glycoprotein

The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell–cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell–cell fusion promoted by two different paramyxovirus F proteins in three cell types in the presence of constitutively active Rho family GTPases, major cellular coordinators of actin dynamics. Reporter gene and syncytia assays demonstrated that expression of either Rac1 V12 or Cdc42 V12 could increase cell–cell fusion promoted by the Hendra or SV5 glycoproteins, though the effect was dependent on the cell type expressing the viral glycoproteins. In contrast, RhoA L63 decreased cell–cell fusion promoted by Hendra glycoproteins but had little affect on SV5 F-mediated fusion. Also, data suggested that GTPase activation in the viral glycoprotein-containing cell was primarily responsible for changes in fusion. Additionally, we found that activated Cdc42 promoted nuclear rearrangement in syncytia.

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Title: Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell–cell fusion
Creators: Rachel M Schowalter - Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509, USA
Mark A Wurth - Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509, USA
Hector C Aguilar - Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA
Benhur Lee - Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA
Carole L Moncman - Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509, USA
Richard O McCann - Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509, USA
Rebecca Ellis Dutch - Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509, USA
Publication Details: Virology (New York, N.Y.), Vol.350(2), pp.323-334
Academic Unit: Paul G. Allen School for Global Animal Health
Publisher: Elsevier Inc
Identifiers: 99900546959801842
Language: English
Resource Type: Journal article