Journal article
Role of Bacterial Communities in the Natural Suppression of Rhizoctonia solani Bare Patch Disease of Wheat (Triticum aestivum L.)
Applied and environmental microbiology, Vol.79(23), pp.7428-7438
12/2013
Handle:
https://hdl.handle.net/2376/110631
PMCID: PMC3837727
PMID: 24056471
Abstract
Rhizoctonia
bare patch and root rot disease of wheat, caused by
Rhizoctonia solani
AG-8, develops as distinct patches of stunted plants and limits the yield of direct-seeded (no-till) wheat in the Pacific Northwest of the United States. At the site of a long-term cropping systems study near Ritzville, WA, a decline in
Rhizoctonia
patch disease was observed over an 11-year period. Bacterial communities from bulk and rhizosphere soil of plants from inside the patches, outside the patches, and recovered patches were analyzed by using pyrosequencing with primers designed for 16S rRNA. Taxa in the class
Acidobacteria
and the genus
Gemmatimonas
were found at higher frequencies in the rhizosphere of healthy plants outside the patches than in that of diseased plants from inside the patches.
Dyella
and
Acidobacteria
subgroup Gp7 were found at higher frequencies in recovered patches.
Chitinophaga
,
Pedobacter
,
Oxalobacteriaceae
(
Duganella
and
Massilia
), and
Chyseobacterium
were found at higher frequencies in the rhizosphere of diseased plants from inside the patches. For selected taxa, trends were validated by quantitative PCR (qPCR), and observed shifts of frequencies in the rhizosphere over time were duplicated in cycling experiments in the greenhouse that involved successive plantings of wheat in
Rhizoctonia
-inoculated soil.
Chryseobacterium soldanellicola
was isolated from the rhizosphere inside the patches and exhibited significant antagonism against
R. solani
AG-8
in vitro
and in greenhouse tests. In conclusion, we identified novel bacterial taxa that respond to conditions affecting bare patch disease symptoms and that may be involved in suppression of
Rhizoctonia
root rot and bare batch disease.
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Details
- Title
- Role of Bacterial Communities in the Natural Suppression of Rhizoctonia solani Bare Patch Disease of Wheat (Triticum aestivum L.)
- Creators
- Chuntao Yin - Department of Plant Pathology, Washington State University, Pullman, Washington, USAScot H Hulbert - Department of Plant Pathology, Washington State University, Pullman, Washington, USAKurtis L Schroeder - Department of Plant Pathology, Washington State University, Pullman, Washington, USAOlga Mavrodi - Department of Plant Pathology, Washington State University, Pullman, Washington, USADmitri Mavrodi - Department of Plant Pathology, Washington State University, Pullman, Washington, USAAmit Dhingra - Department of Horticulture and Landscape Architecture, Washington State University, Pullman, Washington, USAWilliam F Schillinger - Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, USATimothy C Paulitz - USDA Agricultural Research Service, Root Disease and Biological Control Research Unit, Pullman, Washington, USA
- Publication Details
- Applied and environmental microbiology, Vol.79(23), pp.7428-7438
- Academic Unit
- Crop and Soil Sciences, Department of; Horticulture, Department of; Plant Pathology, Department of
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900547368301842
- Language
- English
- Resource Type
- Journal article