Journal article
Role of Ceftiofur in Selection and Dissemination of blaCMY-2-Mediated Cephalosporin Resistance in Salmonella enterica and Commensal Escherichia coli Isolates from Cattle
Applied and environmental microbiology, Vol.75(11), pp.3648-3655
06/2009
Handle:
https://hdl.handle.net/2376/108238
PMCID: PMC2687309
PMID: 19376926
Abstract
Third-generation cephalosporin resistance of Salmonella and commensal Escherichia coli isolates from cattle in the United States is predominantly conferred by the cephamycinase CMY-2, which inactivates β-lactam antimicrobial drugs used to treat a wide variety of infections, including pediatric salmonellosis. The emergence and dissemination of bla CMY-2--bearing plasmids followed and may in part be the result of selection pressure imposed by the widespread utilization of ceftiofur, a third-generation veterinary cephalosporin. This study assessed the potential effects of ceftiofur on bla CMY-2 transfer and dissemination by (i) an in vivo experimental study in which calves were inoculated with competent bla CMY-2-bearing plasmid donors and susceptible recipients and then subjected to ceftiofur selection and (ii) an observational study to determine whether ceftiofur use in dairy herds is associated with the occurrence and frequency of cephalosporin resistance in Salmonella and commensal E. coli. The first study revealed bla CMY-2 plasmid transfer in both ceftiofur-treated and untreated calves but detected no enhancement of plasmid transfer associated with ceftiofur treatment. The second study detected no association (P= 0.22) between ceftiofur use and either the occurrence of ceftiofur-resistant salmonellosis or the frequency of cephalosporin resistance in commensal E. coli. However, herds with a history of salmonellosis (including both ceftiofur-resistant and ceftiofur-susceptible Salmonella isolates) used more ceftiofur than herds with no history of salmonellosis ( P = 0.03) These findings fail to support a major role for ceftiofur use in the maintenance and dissemination of bla CMY-2 -bearing plasmid mediated cephalosporin resistance in commensal E. coli and in pathogenic Salmonella in these dairy cattle populations.
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Details
- Title
- Role of Ceftiofur in Selection and Dissemination of blaCMY-2-Mediated Cephalosporin Resistance in Salmonella enterica and Commensal Escherichia coli Isolates from Cattle
- Creators
- Joshua B Daniels - Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164Douglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164Dale Hancock - Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164William M Sischo - Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164Katherine Baker - Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164Thomas E Besser - Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164
- Publication Details
- Applied and environmental microbiology, Vol.75(11), pp.3648-3655
- Academic Unit
- Department of Veterinary Clinical Sciences; Paul G. Allen School for Global Animal Health; Department of Veterinary Microbiology and Pathology
- Publisher
- American Society for Microbiology (ASM)
- Number of pages
- 8
- Grant note
- 2005-01373 / USDA-NRI Epidemiological Approaches to Food Safety; United States Department of Agriculture (USDA) WSU College of Veterinary Medicine, Pullman, WA N01AI030055 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) N01AI-30055 / NIAID NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Agricultural Animal Health Program
- Identifiers
- 99900547382301842
- Language
- English
- Resource Type
- Journal article