Journal article
Role of the Air-Water-Solid Interface in Bacteriophage Sorption Experiments
Applied and environmental microbiology, Vol.64(1), pp.304-309
01/1998
Handle:
https://hdl.handle.net/2376/104558
PMCID: PMC124709
PMID: 9435082
Abstract
Batch sorption experiments were carried out with the bacteriophages MS2 and φX174. Two types of reactor vessels, polypropylene and glass, were used. Consistently lower concentrations of MS2 were found in the liquid phase in the absence of soil (control blanks) than in the presence of soil after mixing. High levels of MS2 inactivation (∼99.9%) were observed in control tubes made of polypropylene (PP), with comparatively little loss of virus seen in PP tubes when soil was present. Minimal inactivation of MS2 was observed when the air-water interface was completely eliminated from PP control blanks during mixing. All batch experiments performed with reactor tubes made of glass demonstrated no substantial inactivation of MS2. In similar experiments, bacteriophage φX174 did not undergo inactivation in either PP or glass control blanks, implying that this virus is not affected by the same factors which led to inactivation of MS2 in the PP control tubes. When possible, phage adsorption to soil was calculated by the Freundlich isotherm. Our data suggest that forces associated with the air-water-solid interface (where the solid is a hydrophobic surface) are responsible for inactivation of MS2 in the PP control tubes. The influence of air-water interfacial forces should be carefully considered when batch sorption experiments are conducted with certain viruses.
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Details
- Title
- Role of the Air-Water-Solid Interface in Bacteriophage Sorption Experiments
- Creators
- Shawn S Thompson - Department of Soil and Environmental Sciences, University of California, Riverside, California 92521Markus Flury - Department of Soil and Environmental Sciences, University of California, Riverside, California 92521Marylynn V Yates - Department of Soil and Environmental Sciences, University of California, Riverside, California 92521William A Jury - Department of Soil and Environmental Sciences, University of California, Riverside, California 92521
- Publication Details
- Applied and environmental microbiology, Vol.64(1), pp.304-309
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546849001842
- Language
- English
- Resource Type
- Journal article