Journal article
Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of Alternaria alternata
Phytopathology, Vol.100(2), pp.120-126
02/2010
Handle:
https://hdl.handle.net/2376/105494
PMID: 20055645
Abstract
ABSTRACT The tangerine pathotype of Alternaria alternata produces host-selective ACT-toxin and causes Alternaria brown spot disease of tangerines and tangerine hybrids. Sequence analysis of a genomic BAC clone identified a previously uncharacterized portion of the ACT-toxin biosynthesis gene cluster (ACTT). A 1,034-bp gene encoding a putative enoyl-reductase was identified by using rapid amplification of cDNA ends and polymerase chain reaction and designated ACTTS2. Genomic Southern blots demonstrated that ACTTS2 is present only in ACT-toxin producers and is carried on a 1.9 Mb conditionally dispensable chromosome by the tangerine pathotype. Targeted gene disruption of ACTTS2 led to a reduction in ACT-toxin production and pathogenicity, and transcriptional knockdown of ACTTS2 using RNA silencing resulted in complete loss of ACT-toxin production and pathogenicity. These results indicate that ACTTS2 is an essential gene for ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and is required for pathogenicity of this fungus.
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Details
- Title
- Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of Alternaria alternata
- Creators
- Naoya Ajiro - Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, JapanYoko MiyamotoAkira MasunakaTakashi TsugeMikihiro YamamotoKouhei OhtaniTakeshi FukumotoKenji GomiTobin L PeeverYuriko IzumiYasuomi TadaKazuya Akimitsu
- Publication Details
- Phytopathology, Vol.100(2), pp.120-126
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546695101842
- Language
- English
- Resource Type
- Journal article