Journal article
Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
Journal of virology, Vol.77(9), pp.5324-5332
05/2003
Handle:
https://hdl.handle.net/2376/101469
PMCID: PMC153978
PMID: 12692234
Abstract
Herpes simplex virus (HSV) infection of many cultured cells, e.g., Vero cells, can be initiated by receptor binding and pH-neutral fusion with the cell surface. Here we report that a major pathway for HSV entry into the HeLa and CHO-K1 cell lines is dependent on endocytosis and exposure to a low pH. Enveloped virions were readily detected in HeLa or receptor-expressing CHO cell vesicles by electron microscopy at <30 min postinfection. As expected, images of virus fusion with the Vero cell surface were prevalent. Treatment with energy depletion or hypertonic medium, which inhibits endocytosis, prevented uptake of HSV from the HeLa and CHO cell surface relative to uptake from the Vero cell surface. Incubation of HeLa and CHO cells with the weak base ammonium chloride or the ionophore monensin, which elevate the low pH of organelles, blocked HSV entry in a dose-dependent manner. Noncytotoxic concentrations of these agents acted at an early step during infection by HSV type 1 and 2 strains. Entry mediated by the HSV receptor HveA, nectin-1, or nectin-2 was also blocked. As analyzed by fluorescence microscopy, lysosomotropic agents such as the vacuolar H(+)-ATPase inhibitor bafilomycin A1 blocked the delivery of virus capsids to the nuclei of the HeLa and CHO cell lines but had no effect on capsid transport in Vero cells. The results suggest that HSV can utilize two distinct entry pathways, depending on the type of cell encountered.
Metrics
12 Record Views
Details
- Title
- Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
- Creators
- Anthony V Nicola - Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. anicola@niaid.nih.govAnna M McEvoyStephen E Straus
- Publication Details
- Journal of virology, Vol.77(9), pp.5324-5332
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546560401842
- Language
- English
- Resource Type
- Journal article