Journal article
Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae
The EMBO journal, Vol.21(21), pp.5921-5929
11/01/2002
Handle:
https://hdl.handle.net/2376/117649
PMCID: PMC131086
PMID: 12411509
Abstract
Rpb9, a non-essential subunit of RNA polymerase II, mediates a transcription-coupled repair (TCR) subpathway in Saccharomyces cerevisiae. This subpathway initiates at the same upstream site as the previously identified Rad26 subpathway. However, the Rpb9 subpathway operates more effectively in the coding region than in the region upstream of the transcription start site, whereas the Rad26 subpathway operates equally in the two regions. Rpb4, another non-essential subunit of RNA polymerase II, plays a dual role in regulating the two subpathways, suppressing the Rpb9 subpathway and facilitating the Rad26 subpathway. Simultaneous deletion of RPB9 and RAD26 genes completely abolishes TCR in both the coding and upstream regions, indicating that no other TCR subpathway exists in RNA polymerase II-transcribed genes.
Metrics
4 Record Views
Details
- Title
- Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae
- Creators
- Shisheng Li - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman 99164-4660, USAMichael J Smerdon
- Publication Details
- The EMBO journal, Vol.21(21), pp.5921-5929
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- England
- Grant note
- R01 ES004106 / NIEHS NIH HHS ES04106 / NIEHS NIH HHS
- Identifiers
- 99900548094301842
- Language
- English
- Resource Type
- Journal article