Journal article
Ruminant Gastrointestinal Cell Proliferation and Clearance of Escherichia coli O157:H7
Infection and immunity, Vol.68(7), pp.3808-3814
07/2000
Handle:
https://hdl.handle.net/2376/101378
PMCID: PMC101652
PMID: 10858188
Abstract
Human infections with
Escherichia coli
O157:H7 cause hemorrhagic colitis that can progress to a life-threatening sequelae. The most common mode of disease transmission is ingestion of contaminated bovine food products, and it is well established that
E. coli
O157:H7 is a transient member of the bovine microbiota. However, the conditions that induce acquisition and subsequent clearance of this bacterium from the ruminant gastrointestinal tract (GIT) are not understood. Evidence that the rates of epithelial cell proliferation in the lower GIT of cattle are associated with the duration animals remained
E. coli
O157:H7 culture positive is presented. Cattle with slower rates of intestinal cell proliferation in the cecum and the distal colon were culture positive significantly longer than cohort cattle with faster cell proliferation rates. Cell death rates (apoptotic indices) between the short- and long-term culture-positive animals were not different. Typical grain-based finishing diets and forage-based growing diets did not effect GIT cell proliferation or the duration animals remained
E. coli
O157:H7 culture positive. To identify a dietary intervention that would effect GIT cell proliferation, we used sheep as a model ruminant. A fasting-refeeding regime that increased the rate of GIT cell proliferation was developed. The fasting-refeeding protocol was used in cattle to test the hypothesis that feeding interventions that increase the rate of GIT cell proliferation induce the clearance of
E. coli
O157:H7 from the bovine GIT.
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Details
- Title
- Ruminant Gastrointestinal Cell Proliferation and Clearance of Escherichia coli O157:H7
- Creators
- Bernadene A Magnuson - Department of Microbiology, Molecular Biology, and BiochemistryMargaret Davis - Department of Microbiology, Molecular Biology, and BiochemistrySuzanna Hubele - Department of Microbiology, Molecular Biology, and BiochemistryPaula R Austin - Department of Microbiology, Molecular Biology, and BiochemistryIndira T Kudva - Department of Microbiology, Molecular Biology, and BiochemistryChristopher J Williams - Department of Microbiology, Molecular Biology, and BiochemistryCarl W Hunt - Department of Microbiology, Molecular Biology, and BiochemistryCarolyn J Hovde - Department of Microbiology, Molecular Biology, and Biochemistry
- Publication Details
- Infection and immunity, Vol.68(7), pp.3808-3814
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546516001842
- Language
- English
- Resource Type
- Journal article