Journal article
SDF-1/CXCR4 axis modulates bone marrow mesenchymal stem cell apoptosis, migration and cytokine secretion
Protein & cell, Vol.2(10), pp.845-854
10/2011
Handle:
https://hdl.handle.net/2376/106971
PMCID: PMC4875294
PMID: 22058039
Abstract
Bone marrow mesenchymal stem cells (MSCs) are considered as a promising cell source to treat the acute myocardial infarction. However, over 90% of the stem cells usually die in the first three days of transplantation. Survival potential, migration ability and paracrine capacity have been considered as the most important three factors for cell transplantation in the ischemic cardiac treatment. We hypothesized that stromal-derived factor-1 (SDF-1)/CXCR4 axis plays a critical role in the regulation of these processes. In this study, apoptosis was induced by exposure of MSCs to H
2
O
2
for 2 h. After re-oxygenation, the SDF-1 pretreated MSCs demonstrated a significant increase in survival and proliferation. SDF-1 pretreatment also enhanced the migration and increased the secretion of pro-survival and angiogenic cytokines including basic fibroblast growth factor and vascular endothelial growth factor. Western blot and RT-PCR demonstrated that SDF-1 pretreatment significantly activated the pro-survival Akt and Erk signaling pathways and up-regulated Bcl-2/Bax ratio. These protective effects were partially inhibited by AMD3100, an antagonist of CXCR4.We conclude that the SDF-1/CXCR4 axis is critical for MSC survival, migration and cytokine secretion.
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Details
- Title
- SDF-1/CXCR4 axis modulates bone marrow mesenchymal stem cell apoptosis, migration and cytokine secretion
- Creators
- Xiaolei Liu - Tianjin, 300071 ChinaBiyan Duan - Tianjin, 300071 ChinaZhaokang Cheng - Tianjin, 300071 ChinaXiaohua Jia - Tianjin, 300071 ChinaLina Mao - Tianjin, 300071 ChinaHao Fu - Tianjin, 300071 ChinaYongzhe Che - Tianjin, 300071 ChinaLailiang Ou - Tianjin, 300071 ChinaLin Liu - Tianjin, 300071 ChinaDeling Kong - Tianjin, 300071 China
- Publication Details
- Protein & cell, Vol.2(10), pp.845-854
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Higher Education Press; Beijing
- Identifiers
- 99900546608701842
- Language
- English
- Resource Type
- Journal article