Files and links (2)
CC BY V4.0, Open Access
Journal article
Safety and Immunogenicity of a Mycoplasma ovipneumoniae bacterin for domestic sheep (Ovis aries)
PloS one, Vol.9(4), pp.e95698-e95698
2014
Handle:
https://hdl.handle.net/2376/116040
PMCID: PMC3994082
PMID: 24752006
Abstract
Mortality from epizootic pneumonia is hindering re-establishment of bighorn sheep populations in western North America. Mycoplasma ovipneumoniae, a primary agent of this disease, is frequently carried asymptomatically by the domestic sheep and goats that constitute the reservoir of this agent for transmission to bighorn sheep. Our long-term objective is to reduce the risk of M. ovipneumoniae infection of bighorn sheep; one approach to this objective is to control the pathogen in its reservoir hosts.
The safety and immunogenicity of M. ovipneumoniae for domestic sheep was evaluated in three experimental immunization protocols: 1) live M. ovipneumoniae (50 ug protein); 2) killed M. ovipneumoniae (50 ug whole cell protein) in oil adjuvant; and 3) killed M. ovipneumoniae (250 ug whole cell protein) in oil adjuvant. Immunogenicity was assessed by two serum antibody measures: competitive enzyme-linked immunosorbent assay (cELISA) (experiments 1-3) and serum growth inhibition (Experiment 3). Passive immunogenicity was also assessed in the third experiment using the same assays applied to blood samples obtained from the lambs of immunized ewes.
Adverse reactions to immunization were generally minor, but local reactions were regularly observed at immunization sites with bacterins in oil adjuvants. No evidence of M. ovipneumoniae specific antibody responses were observed in the first or second experiments and no resistance to colonization was observed in the first experiment. However, the ewes in the third experiment developed strong cELISA serum antibody responses and significant serum M. ovipneumoniae inhibition activity, and these responses were passively transferred to their lambs. The results of these trials indicate that immunization with relatively large antigenic mass combined with an adjuvant is capable of inducing strong active antibody responses in ewes and passively immunizing lambs.
Metrics
19 Record Views
Details
- Title
- Safety and Immunogenicity of a Mycoplasma ovipneumoniae bacterin for domestic sheep (Ovis aries)
- Creators
- Jessie C Ziegler - Department of Veterinary Clinical Medicine, Washington State University, Pullman, Washington, United States of AmericaKevin K Lahmers - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of AmericaGeorge M Barrington - Department of Veterinary Clinical Medicine, Washington State University, Pullman, Washington, United States of AmericaSteven M Parish - Department of Veterinary Clinical Medicine, Washington State University, Pullman, Washington, United States of AmericaKatherine Kilzer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of AmericaKatherine Baker - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of AmericaThomas E Besser - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America
- Publication Details
- PloS one, Vol.9(4), pp.e95698-e95698
- Academic Unit
- Paul G. Allen School for Global Animal Health; Department of Veterinary Microbiology and Pathology
- Publisher
- United States
- Identifiers
- 99900548186501842
- Language
- English
- Resource Type
- Journal article