Journal article
Salmonella Enteritidis Strains from Poultry Exhibit Differential Responses to Acid Stress, Oxidative Stress, and Survival in the Egg Albumen
Foodborne pathogens and disease, Vol.9(3), pp.258-264
03/01/2012
Handle:
https://hdl.handle.net/2376/109077
PMCID: PMC3326446
PMID: 22304629
Abstract
Salmonella
Enteritidis is the major foodborne pathogen that is primarily transmitted by contaminated chicken meat and eggs. We recently demonstrated that
Salmonella
Enteritidis strains from poultry differ in their ability to invade human intestinal cells and cause disease in orally challenged mice. Here we hypothesized that the differential virulence of
Salmonella
Enteritidis strains is due to the differential fitness in the adverse environments that may be encountered during infection in the host. The responses of a panel of six
Salmonella
Enteritidis strains to acid stress, oxidative stress, survival in egg albumen, and the ability to cause infection in chickens were analyzed. This analysis allowed classification of strains into two categories, stress-sensitive and stress-resistant, with the former showing significantly (
p
<0.05) reduced survival in acidic (gastric phase of infection) and oxidative (intestinal and systemic phase of infection) stress. Stress-sensitive strains also showed impaired intestinal colonization and systemic dissemination in orally inoculated chickens and failed to survive/grow in egg albumen. Comparative genomic hybridization microarray analysis revealed no differences at the discriminatory level of the whole gene content between stress-sensitive and stress-resistant strains. However, sequencing of
rpoS,
a stress-regulatory gene, revealed that one of the three stress-sensitive strains carried an insertion mutation in the
rpoS
resulting in truncation of σ
S
. Finding that one of the stress-sensitive strains carried an easily identifiable small polymorphism within a stress-response gene suggests that the other strains may also have small polymorphisms elsewhere in the genome, which likely impact regulation of stress or virulence associated genes in some manner.
Metrics
5 Record Views
Details
- Title
- Salmonella Enteritidis Strains from Poultry Exhibit Differential Responses to Acid Stress, Oxidative Stress, and Survival in the Egg Albumen
- Creators
- Devendra H Shah - 2The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WashingtonCarol Casavant - 1Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WashingtonQuincy Hawley - 3College of Veterinary Medicine, North Carolina State University, Raleigh, North CarolinaTarek Addwebi - 1Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WashingtonDouglas R Call - 2The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WashingtonJean Guard - 4Egg Quality and Safety Research Unit, Agriculture Research Service, United States Department of Agriculture, Athens, Georgia
- Publication Details
- Foodborne pathogens and disease, Vol.9(3), pp.258-264
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Mary Ann Liebert, Inc
- Identifiers
- 99900547043401842
- Language
- English
- Resource Type
- Journal article