Sclerotinia sclerotiorum Populations Infecting Canola from China and the United States Are Genetically and Phenotypically Distinct

Renuka N ATTANAYAKE; Patrick A CARTER; DAOHONG JIANG; Luis DEL RIO-MENDOZA; WEIDONG WEIDONG CHEN

doi:10.1094/PHYTO-07-12-0159-R

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Sclerotinia sclerotiorum Populations Infecting Canola from China and the United States Are Genetically and Phenotypically Distinct

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Sclerotinia sclerotiorum Populations Infecting Canola from China and the United States Are Genetically and Phenotypically Distinct

Renuka N ATTANAYAKE, Patrick A CARTER, DAOHONG JIANG, Luis DEL RIO-MENDOZA and WEIDONG WEIDONG CHEN

Phytopathology, Vol.103(7), pp.750-761

2013

DOI: https://doi.org/10.1094/PHYTO-07-12-0159-R

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https://hdl.handle.net/2376/103536

PMID: 23464902

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Abstract

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Phytopathology. Animal pests. Plant and forest protection

Fungal plant pathogens

Genetic and phenotypic diversity and population differentiation of Sclerotinia sclerotiorum isolates infecting canola from China and the United States were investigated. Genetic diversity was assessed with eight microsatellite markers and mycelial compatibility groups (MCGs). Phenotypic diversity was assessed with sensitivity to three fungicides, production of oxalate and sclerotia, growth rate, and virulence on two canola cultivars. No shared MCGs or multilocus haplotypes were detected between the two populations, and populations differed significantly (P < 0.001). Recombination was detected in both populations but was greater in the Chinese population. A polymerase chain reaction detection assay showed that ~60% of the isolates were inversion-plus at the mating type locus. The two populations differed significantly (P < 0.05) for all of the phenotypic traits except for sensitivity to fungicide fluazinam and virulence. Isolates in the Chinese population were unique in several aspects. Despite the phenotypic differentiation, heritabilities of the pheno-typic traits were similar for both populations. Significant correlations were found among five phenotypic traits. Cross resistance to benomyl and iprodione was detected. Virulence was not significantly correlated with any other phenotypic trait and had the least heritability. However, both populations were equally virulent on either a susceptible or a moderately resistant canola cultivars.

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Title: Sclerotinia sclerotiorum Populations Infecting Canola from China and the United States Are Genetically and Phenotypically Distinct
Creators: Renuka N ATTANAYAKE - Department of Plant Pathology, and second author: School of Biological Sciences, Washington State University, Pullman 99164, United States
Patrick A CARTER - Department of Plant Pathology, and second author: School of Biological Sciences, Washington State University, Pullman 99164, United States
DAOHONG JIANG - Department of Plant Protection, Huazhong Agricultural University, Wuhan, Hubei, China
Luis DEL RIO-MENDOZA - Department of Plant Pathology, North Dakota State University, Fargo 58108, United States
WEIDONG WEIDONG CHEN - Washington State University, Department of Plant Pathology
Publication Details: Phytopathology, Vol.103(7), pp.750-761
Academic Unit: School of Biological Sciences
Publisher: American Phytopathological Society; St. Paul, MN
Identifiers: 99900546530801842
Language: English
Resource Type: Journal article