Journal article
Screening and characterization of aldehyde dehydrogenase gene from Halomonas salina strain AS11
Journal of biotechnology, Vol.95(2), pp.171-179
2002
Handle:
https://hdl.handle.net/2376/114245
PMID: 11911926
Abstract
A population survey was made of moderately halophilic bacteria in prawn pond sediment in the Songkla region of Thailand. Twenty-two isolated halophilic bacteria capable of growing on modified ATCC culture medium 1270 for halobacterium were then assayed for aldehyde dehydrogenase (ALDH) activity which might be involved in the metabolism of xenobiotic compounds. One isolate, designated AS11, was selected based on its high amount of ALDH activity. This organism can grow at sodium chloride concentrations ranging from 2.5 to 25%, although optimum growth occurs at 5% NaCl. Phenotypic and phylogenetic studies indicated that AS11 was an isolate of
Halomonas salina. The
aldh gene coding for this enzyme was then cloned. The open reading frame of the
aldh gene was 1521-bp long and coded for a protein of 506 amino acid residues with a calculated molecular mass of 55 kDa. The
aldh gene product proved to be 76% identical to the NAD-dependent acetaldehyde dehydrogenase gene from
Pseudomonase aeruginosa.
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Details
- Title
- Screening and characterization of aldehyde dehydrogenase gene from Halomonas salina strain AS11
- Creators
- Thanya Sripo - Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hatyai, Songkla 90112, ThailandAmornrat Phongdara - Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hatyai, Songkla 90112, ThailandChokchai Wanapu - School of Biotechnology, Institute of Agriculture Technology, Suranaree University of Technology, 111 University Avenue, Maung, Nakon Ratchasrima 30000, ThailandAllan B Caplan - Department of Microbiology, Molecular Biology and Biochemistry, College of Agriculture, University of Idaho, Moscow, ID 83844-3052, USA
- Publication Details
- Journal of biotechnology, Vol.95(2), pp.171-179
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier B.V
- Identifiers
- 99900547933201842
- Language
- English
- Resource Type
- Journal article