Journal article
Screening of antidote sensitivity using an acetylcholinesterase biosensor based on a graphene-Au nanocomposite
RSC advances, Vol.5(7), pp.4894-4897
01/01/2015
Abstract
In this paper, we developed a new technique for in vitro screening of the therapeutic effects of three oxime compounds: obidoxime, 2-pralidoxime methiodide (2-PAM-I) and pyridine-2-aldoxime methochloride (2-PAM-Cl). Acetylcholinesterase (AChE) is first immobilized in a thin nanocomposite film composed of graphene and gold nanoparticles on a glassy carbon electrode by a self-assembly approach. The immobilized AChE in the nanocomposite is first exposed to an organophosphate agent, paraoxon-ethyl, and the enzyme activity is inhibited. The therapeutic effect is based on the reactivation capability of the oximes. AChE activity is measured by an electrochemical method. Our study indicated that oximes reactivation capacity is correlated to their molecular structures. Obidoxime, having dual oxime groups, showed higher reactivation capacity than 2-PAM compounds with a single oxime group. Due to the similar molecular structures of 2-PAM-I and 2-PAM-Cl, a slight difference between their reactivation capacities was found, and this difference is attributed to the stronger electron withdrawing ability of iodine ions than that of chloride ions. The proposed electrochemical method thus provides a new simple tool for new drug screening.
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Details
- Title
- Screening of antidote sensitivity using an acetylcholinesterase biosensor based on a graphene-Au nanocomposite
- Creators
- Linjuan Long - Central China Normal UniversityYanan Luo - Central China Normal UniversityBingwen Liu - Washington State UniversityDan Du - Washington State UniversityYuehe Lin - Washington State University
- Publication Details
- RSC advances, Vol.5(7), pp.4894-4897
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Royal Soc Chemistry
- Number of pages
- 4
- Grant note
- 21275062 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) NCET-12-0871 / Program for New Century Excellent Talents in University; Program for New Century Excellent Talents in University (NCET) WSU start-up grant
- Identifiers
- 99901227637601842
- Language
- English
- Resource Type
- Journal article