Journal article
Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass
Canadian journal of microbiology, Vol.61(10), pp.745-752
2015
Handle:
https://hdl.handle.net/2376/108195
PMID: 26286682
Abstract
To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxidase activity and no lignin peroxidase activity were detected. Strain BBEL0970 proved to be the most efficient in laccase production and was subsequently identified as Trametes versicolor by analysis of the ribosomal DNA internal transcribed spacer gene sequence. In combining laccase production with biological pretreatment, the replacement of glucose with barley straw significantly improved the laccase activity by up to 10.3 U/mL, which provided evidence toward potential utilization of barley straw in laccase production by BBEL0970. Simultaneously, comparison by thermogravimetric analysis of the untreated and pretreated barley straw in liquid fermentation of laccase also demonstrated the high potential of BBEL0970 in biological pretreatment of lignocellulosic biomass. This work sheds light on further exploration on the integrated process of low-cost laccase production and efficient biological pretreatment of barley straw by T. versicolor BBEL0970.
Metrics
11 Record Views
Details
- Title
- Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass
- Creators
- Chunyan Xu - Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan 750021, People’s Republic of ChinaDeepak Singh - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USAKathleen M Dorgan - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USAXiaoyu Zhang - Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People’s Republic of ChinaShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Canadian journal of microbiology, Vol.61(10), pp.745-752
- Publisher
- NRC Research Press
- Identifiers
- 99900582336901842
- Language
- English
- Resource Type
- Journal article