Journal article
Secondary Structure and Dynamics of an Intrinsically Unstructured Linker Domain
Journal of biomolecular structure & dynamics, Vol.23(2), pp.113-124
10/01/2005
Handle:
https://hdl.handle.net/2376/106796
PMID: 16060685
Abstract
The transient secondary structure and dynamics of an intrinsically unstructured linker domain from the 70 kDa subunit of human replication protein A was investigated using solution state NMR. Stable secondary structure, inferred from large secondary chemical shifts, was observed for a segment of the intrinsically unstructured linker domain when it is attached to an N-terminal protein interaction domain. Results from NMR relaxation experiments showed the rotational diffusion for this segment of the intrinsically unstructured linker domain to be correlated with the N-terminal protein interaction domain. When the N-terminal domain is removed, the stable secondary structure is lost and faster rotational diffusion is observed. The large secondary chemical shifts were used to calculate phi and psidihedral angles and these dihedral angles were used to build a backbone structural model. Restrained molecular dynamics were performed on this new structure using the chemical shift based dihedral angles and a single NOE distance as restraints. In the resulting family of structures a large, solvent exposed loop was observed for the segment of the intrinsically unstructured linker domain that had large secondary chemical shifts.
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Details
- Title
- Secondary Structure and Dynamics of an Intrinsically Unstructured Linker Domain
- Creators
- Katie E Olson - Department of Microbiology, Molecular Biology, and Biochemistry , University of IdahoPranesh Narayanaswami - Department of Microbiology, Molecular Biology, and Biochemistry , University of IdahoPamela D Vise - Department of Microbiology, Molecular Biology, and Biochemistry , University of IdahoDavid F Lowry - Department of Microbiology, Molecular Biology, and Biochemistry , University of IdahoMarc S Wold - Department of Biochemistry , University of Iowa College of MedicineGary W Daughdrill - Department of Microbiology, Molecular Biology, and Biochemistry , University of Idaho
- Publication Details
- Journal of biomolecular structure & dynamics, Vol.23(2), pp.113-124
- Academic Unit
- Engineering and Applied Sciences (TRIC), School of
- Publisher
- Taylor & Francis Group
- Identifiers
- 99900546864101842
- Language
- English
- Resource Type
- Journal article