Journal article
Secondary and quaternary structures of the (+)-pinoresinol-forming dirigent protein
Biochemistry (Easton), Vol.41(30), pp.9455-9461
07/30/2002
Handle:
https://hdl.handle.net/2376/110136
PMID: 12135368
Abstract
The (+)-pinoresinol-forming dirigent protein is the first protein capable of stereoselectively coupling two coniferyl alcohol derived radical species, in this case to give the 8-8' linked (+)-pinoresinol. Only dimeric cross-linked dirigent protein structures were isolated when 1-ethyl-3-[3-(dimethylamino)-propyl]carbodiimide was used as cross-linking agent, whereas the associated oxidase, presumed to generate the corresponding free radical substrate, was not detected. Native Forsythia intermedia dirigent protein isoforms were additionally subjected to MALDI-TOF and ESI-MS analyses, which established the presence of both monomeric masses of 23-25 kDa and dimeric dirigent protein species ranging from 46 to 49 kDa. Analytical ultracentrifugation, sedimentation velocity, and sedimentation equilibrium analyses of the native dirigent protein in open solution confirmed further its dimeric nature as well as a propensity to aggregate, with the latter being dependent upon both temperature and solution ionic strength. Circular dichroism analysis suggested that the dirigent protein was primarily composed of beta-sheet and loop structures.
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Details
- Title
- Secondary and quaternary structures of the (+)-pinoresinol-forming dirigent protein
- Creators
- Steven C Halls - Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USANorman G Lewis
- Publication Details
- Biochemistry (Easton), Vol.41(30), pp.9455-9461
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- United States
- Identifiers
- 99900546923001842
- Language
- English
- Resource Type
- Journal article