Journal article
Secreted Euryarchaeal Microhalocins Kill Hyperthermophilic Crenarchaea
Journal of bacteriology, Vol.183(1), pp.287-291
01/2001
Handle:
https://hdl.handle.net/2376/102810
PMCID: PMC94877
PMID: 11114928
Abstract
Few antibiotics targeting members of the archaeal domain are currently available for genetic studies. Since bacterial antibiotics are frequently directed against competing and related organisms, archaea by analogy might produce effective antiarchaeal antibiotics. Peptide antibiotic (halocin) preparations from euryarchaeal halophilic strains S8a, GN101, and TuA4 were found to be toxic for members of the hyperthermophilic crenarchaeal genus
Sulfolobus
. No toxicity was evident against representative bacteria or eukarya. Halocin S8 (strain S8a) and halocin R1 (strain GN101) preparations were cytostatic, while halocin A4 (strain TuA4) preparations were cytocidal. Subsequent studies focused on the use of halocin A4 preparations and
Sulfolobus solfataricus
. Strain TuA4 cell lysates were not toxic for
S. solfataricus
, and protease (but not nuclease) treatment of the halocin A4 preparation inactivated toxicity, indicating that the A4 toxic factor must be a secreted protein. Potassium chloride supplementation of the
Sulfolobus
assay medium potentiated toxicity, implicating use of a salt-dependent mechanism. The utility of halocin A4 preparations for genetic manipulation of
S. solfataricus
was assessed through the isolation of UV-induced resistant mutants. The mutants exhibited stable phenotypes and were placed into distinct classes based on their levels of resistance.
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Details
- Title
- Secreted Euryarchaeal Microhalocins Kill Hyperthermophilic Crenarchaea
- Creators
- Cynthia Haseltine - University of Nebraska, Lincoln, NebraskaTiffany Hill - University of Nebraska, Lincoln, NebraskaRafael Montalvo-Rodriguez - University of Nebraska, Lincoln, NebraskaSamantha K Kemper - University of Nebraska, Lincoln, NebraskaRichard F Shand - University of Nebraska, Lincoln, NebraskaPaul Blum - University of Nebraska, Lincoln, Nebraska
- Publication Details
- Journal of bacteriology, Vol.183(1), pp.287-291
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546506801842
- Language
- English
- Resource Type
- Journal article