Journal article
Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus
Journal of bacteriology, Vol.186(11), pp.3296-3303
06/2004
Handle:
https://hdl.handle.net/2376/116414
PMCID: PMC415756
PMID: 15150214
Abstract
Campylobacter jejuni
, a gram-negative motile bacterium, secretes a set of proteins termed the
Campylobacter
invasion antigens (Cia proteins). The purpose of this study was to determine whether the flagellar apparatus serves as the export apparatus for the Cia proteins. Mutations were generated in five genes encoding three structural components of the flagella, the flagellar basal body (
flgB
and
flgC
), hook (
flgE2
), and filament (
flaA
and
flaB
) genes, as well as in genes whose products are essential for flagellar protein export (
flhB
and
fliI
). While mutations that affected filament assembly were found to be nonmotile (Mot
−
) and did not secrete Cia proteins (S
−
), a
flaA
(
flaB
+
) filament mutant was found to be nonmotile but Cia protein secretion competent (Mot
−
, S
+
). Complementation of a
flaA flaB
double mutant with a shuttle plasmid harboring either the
flaA
or
flaB
gene restored Cia protein secretion, suggesting that Cia export requires at least one of the two filament proteins. Infection of INT 407 human intestinal cells with the
C. jejuni
mutants revealed that maximal invasion of the epithelial cells required motile bacteria that are secretion competent. Collectively, these data suggest that the
C. jejuni
Cia proteins are secreted from the flagellar export apparatus.
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Details
- Title
- Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus
- Creators
- Michael E Konkel - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164John D Klena - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Vanessa Rivera-Amill - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Marshall R Monteville - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Debabrata Biswas - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Brian Raphael - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Joey Mickelson - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
- Publication Details
- Journal of bacteriology, Vol.186(11), pp.3296-3303
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547698701842
- Language
- English
- Resource Type
- Journal article