Journal article
Secretome characteristics of pelletized Trichoderma reesei and cellulase production
World journal of microbiology & biotechnology, Vol.28(8), pp.2635-2641
08/2012
Handle:
https://hdl.handle.net/2376/108164
PMID: 22806189
Abstract
Trichoderma reesei is an important cellulase producer and its secondary mycelial phase is responsible for cellulase expression and secretion in submerged fermentation. Little is known regarding the effects of fungal morphology on cellulase production by Trichoderma sp. In this study we aimed to extend the understanding of cellulase production by T. reesei, especially correlating cellulase productivity with pellet morphology and with its secretome characteristics. We found that T. reesei was more likely to form pellets in malt extract broth than in potato dextrose broth. CaCO3 helped in formation of fine pellets in malt extract broth. 109 spores/ml resulted in formation of pellets with the size of 0.13 ± 0.047 mm. LC/MS spectrometry analysis indicated that the secretomes from pellet was different from that of mycelial mat under the same fermentation conditions. Optimization tests showed that lactose, xylose and Pluronic F68 are important for efficient production of cellulases with FPU activity in the pellets fermentation. This is the first report on the artificial formation of pellets by Trichoderma sp. as well as correlation between physiological characteristic of the pellets and cellulase production by T. reesei. The findings from this study can be used for improvement of cellulase productivity.
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Details
- Title
- Secretome characteristics of pelletized Trichoderma reesei and cellulase production
- Creators
- Yapeng Chao - State Key Laboratories of Transducer Technology, Institute of Microbiology Chinese Academy of Sciences Beijing 100101 ChinaDeepak Singh - Department of Biological System Engineering Washington State University Pullman WA 99164 USALiang Yu - Department of Biological System Engineering Washington State University Pullman WA 99164 USAZhiming Li - Department of Biological System Engineering Washington State University Pullman WA 99164 USAZhanyou Chi - Department of Biological System Engineering Washington State University Pullman WA 99164 USAShulin Chen - Department of Biological System Engineering Washington State University Pullman WA 99163 USA
- Publication Details
- World journal of microbiology & biotechnology, Vol.28(8), pp.2635-2641
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547033801842
- Language
- English
- Resource Type
- Journal article