Journal article
Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene
Immunology, Vol.76(2), pp.305-309
06/1992
Handle:
https://hdl.handle.net/2376/109588
PMCID: PMC1421522
PMID: 1634253
Abstract
The bovine interleukin-2 receptor-alpha (IL-2R alpha) gene has been isolated and a rabbit antiserum against a fusion protein of the gene has been produced. However, the antiserum does not inhibit IL-2-dependent proliferation. Since a panel of monoclonal antibodies (mAb) to bovine activation antigens was available, we transfected the gene into mouse L fibroblasts, selected stable transfectants with the rabbit antiserum, and screened for antibodies that bound the transfected cells but not the untransfected cells. Three mAb were selected and all three precipitated a molecule of M(r) 55,000 (under reducing conditions) from activated cells, as expected from homology with mouse and human IL-2R alpha (CD25, Tac). One of the three mAb was a strong inhibitor of IL-2-dependent proliferation of bovine lymphocytes. Thus, the availability of transfected cells allowed us to establish quickly and unequivocally the antigenic specificity of a number of antibodies.
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Details
- Title
- Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene
- Creators
- J Naessens - ILRAD, Nairobi, KenyaM Sileghem - ILRAD, Nairobi, KenyaN MacHugh - ILRAD, Nairobi, KenyaY H Park - ILRAD, Nairobi, KenyaW C Davis - ILRAD, Nairobi, KenyaP Toye - ILRAD, Nairobi, Kenya
- Publication Details
- Immunology, Vol.76(2), pp.305-309
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Identifiers
- 99900547284001842
- Language
- English
- Resource Type
- Journal article