Journal article
Selection on Glycine β-1,3-Endoglucanase Genes Differentially Inhibited by a Phytophthora Glucanase Inhibitor Protein
Genetics (Austin), Vol.169(2), pp.1009-1019
02/2005
Handle:
https://hdl.handle.net/2376/101607
PMCID: PMC1449112
PMID: 15545660
Abstract
Plant endo-β-1,3-glucanases (EGases) degrade the cell wall polysaccharides of attacking pathogens and release elicitors of additional plant defenses. Isozymes EGaseA and EGaseB of soybean differ in susceptibility to a glucanase inhibitor protein (GIP1) produced by
Phytophthora sojae
, a major soybean pathogen. EGaseA, the major elicitor-releasing isozyme, is a high-affinity ligand for GIP1, which completely inhibits it, whereas EGaseB is unaffected by GIP1. We tested for departures from neutral evolution on the basis of partial sequences of
EGaseA
and
EGaseB
from 20 widespread accessions of
Glycine soja
(the wild progenitor of soybean), from 4 other Glycine species, and across dicotyledonous plants.
G. soja
exhibited little intraspecific variation at either locus. Phylogeny-based codon evolution models detected strong evidence of positive selection on Glycine EGaseA and weaker evidence for selection on dicot EGases and Glycine EGaseB. Positively selected peptide sites were identified and located on a structural model of EGase bound to GIP1. Positively selected sites and highly variable sites were found disproportionately within 4.5 Å of bound GIP1. Low variation within
G. soja
EGases, coupled with positive selection in both Glycine and dicot lineages and the proximity of rapidly evolving sites to GIP1, suggests an arms race involving repeated adaptation to pathogen attack and inhibition.
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Details
- Title
- Selection on Glycine β-1,3-Endoglucanase Genes Differentially Inhibited by a Phytophthora Glucanase Inhibitor Protein
- Creators
- J. G Bishop - School of Biological Sciences, Washington State University, Vancouver, Washington 98686-9600D. R Ripoll - Computational Biology Service Unit, Cornell Theory CenterS Bashir - Department of Plant Biology, Cornell University, Ithaca, New York 14853C. M. B Damasceno - Department of Plant Biology, Cornell University, Ithaca, New York 14853J. D Seeds - School of Biological Sciences, Washington State University, Vancouver, Washington 98686-9600J. K. C Rose - Department of Plant Biology, Cornell University, Ithaca, New York 14853
- Publication Details
- Genetics (Austin), Vol.169(2), pp.1009-1019
- Academic Unit
- Biological Sciences, School of
- Publisher
- Genetics Society of America
- Identifiers
- 99900546518501842
- Language
- English
- Resource Type
- Journal article