Journal article
Self-Assembly of All-Inclusive Allochroic Nanoparticles for the Improved ELISA
Analytical chemistry (Washington), Vol.91(13), pp.8461-8465
07/02/2019
PMID: 31144813
Abstract
Improvement in the sensitivity and portability of enzyme-linked immunosorbent assay (ELISA) still faced huge challenges recently. Inspired by the nanoprecipitation process for the carrier-free nanodrug in drug delivery systems, we developed an self-assembly strategy to prepare a kind of all-inclusive allochroic 3,3',5,5'-tetramethylbenzidine (TMB) nanoparticles for developing improved ELISA (named TLISA). During the self-assembly of TMB molecules, the antibody as signal recognition units and bovine serum albumin as the stabilizing agents could be accommodated simultaneously through hydrogen-bonding and van der Waals interactions to form all-inclusive signal tags, which can avoid the complex cross-linking procedures. Due to the ultrahigh loading capacity (817 mg/g) of TMB molecules, the sensitivity of TLISA toward inflammatory biomarkers interleukin-6 was 11.82 times higher than that of conventional ELISA, which paves an avenue to develop an ultrasensitive colorimetric immunoassay.
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Details
- Title
- Self-Assembly of All-Inclusive Allochroic Nanoparticles for the Improved ELISA
- Creators
- Lei Jiao - Central China Normal UniversityHongye Yan - Central China Normal UniversityWeiqing Xu - Central China Normal UniversityYu Wu - Central China Normal UniversityWenling Gu - Central China Normal UniversityHe Li - Chengdu University of Information TechnologyDan Du - Washington State UniversityYuehe Lin - School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United StatesChengzhou Zhu - Central China Normal University
- Publication Details
- Analytical chemistry (Washington), Vol.91(13), pp.8461-8465
- Academic Unit
- School of Mechanical and Materials Engineering
- Identifiers
- 99901227644401842
- Language
- English
- Resource Type
- Journal article