Journal article
Self assembly of acetylcholinesterase on a gold nanoparticles-graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker
Journal of materials chemistry, Vol.21(14), pp.5319-5325
01/01/2011
Abstract
A nanohybrid of gold nanoparticles (Au NPs) and chemically reduced graphene oxide nanosheets (cr-Gs) was synthesized by in situ growth of Au NPs on the surface of graphene nanosheets in the presence of poly(diallyldimethylammonium chloride) (PDDA), which not only improved the dispersion of Au NPs but also stabilized cholinesterase with high activity and loading efficiency. The obtained nanohybrid was characterized by TEM, XRD, XPS, and electrochemistry. Then an enzyme nanoassembly (AChE/Au NPs/cr-Gs) was prepared by self-assembling acetylcholinesterase (AChE) on Au NP/cr-Gs nanohybrid. An electrochemical sensor based on AChE/Au NPs/cr-Gs was further developed for ultrasensitive detection of organophosphate pesticide. The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.
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Details
- Title
- Self assembly of acetylcholinesterase on a gold nanoparticles-graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker
- Creators
- Ying Wang - Tsinghua UniversitySheng Zhang - Pacific Northwest National LaboratoryDan Du - Pacific Northwest National LaboratoryYuyan Shao - Pacific Northwest National LaboratoryZhaohui Li - Pacific Northwest National LaboratoryJun Wang - Pacific Northwest National LaboratoryMark H. Engelhard - Pacific Northwest National LaboratoryJinghong Li - Tsinghua UniversityYuehe Lin - Pacific Northwest National Laboratory
- Publication Details
- Journal of materials chemistry, Vol.21(14), pp.5319-5325
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Royal Soc Chemistry
- Number of pages
- 7
- Grant note
- Pacific Northwest National Laboratory (PNNL) DOE's office of Biological and Environmental Research located at PNNL; United States Department of Energy (DOE) 200950431184 / Program for Chenguang Young Scientist for Wuhan NS058161-01 / National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) 2007CB310500 / National Basic Research Program of China 20975060; 21075047 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) DE-AC05-76RL01830 / U.S. Department of Energy (DOE); United States Department of Energy (DOE)
- Identifiers
- 99901227849701842
- Language
- English
- Resource Type
- Journal article