Seminal transferrin and spermatogenic capability in the bull

R R Gilmont; P L Senger; S R Sylvester; M D Griswold

doi:10.1095/biolreprod43.1.151

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Seminal transferrin and spermatogenic capability in the bull

Journal article

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Seminal transferrin and spermatogenic capability in the bull

R R Gilmont, P L Senger, S R Sylvester and M D Griswold

Biology of reproduction, Vol.43(1), pp.151-157

07/1990

DOI: https://doi.org/10.1095/biolreprod43.1.151

Handle:

https://hdl.handle.net/2376/110536

PMID: 2393688

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Abstract

Transferrin - analysis

Regression Analysis

Sperm Count

Spermatogenesis - physiology

Animals

Semen - analysis

Radioimmunoassay

Male

Transferrin - physiology

Cattle - physiology

The objective of this study was to determine the relationship between seminal transferrin and sperm output in ejaculates from mature dairy bulls. Caudal sperm reserves in mature Holstein bulls (n = 15) were depleted by 8 successive ejaculations during a 50-70-min period (depletion phase). Bulls were then ejaculated 6 times per week for a period of 4 weeks (6X phase). Weekly sperm output (WSO) and weekly transferrin output (WTfO) were the sums of sperm and transferrin levels in 6 ejaculates taken in 1 week of the study. Mean WSO ranged from 20.7 billion to 39.6 billion and mean WTfO ranged from 334 micrograms to 1872 micrograms among the bulls. Regression analysis of sperm and transferrin levels in ejaculates collected during the depletion phase indicated that approximately 40% of seminal transferrin was not related to sperm output and probably was from accessory fluids. A relationship between total seminal transferrin and total sperm in ejaculate was observed (p less than 0.01, r = 0.54). This relationship was stronger when the transferrin was corrected for accessory fluid contribution (p less than 0.01, r = 0.65). The relationship between WSO and WTfO corrected for accessory fluid transferrin contribution (cWTfO) was significant (p less than 0.01, r = 0.64). The relationship between WSO and cWTfO can be interpreted to reflect the relationship between actual testicular sperm production and transferrin from testicular or epididymal origin.

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Title: Seminal transferrin and spermatogenic capability in the bull
Creators: R R Gilmont - Genetics and Cell Biology Program, Washington State University, Pullman 99164-4660
P L Senger
S R Sylvester
M D Griswold
Publication Details: Biology of reproduction, Vol.43(1), pp.151-157
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: HD-10808 / NICHD NIH HHS
Identifiers: 99900547397301842
Language: English
Resource Type: Journal article