Journal article
Sensitive Immunosensor for Cancer Biomarker Based on Dual Signal Amplification Strategy of Graphene Sheets and Multienzyme Functionalized Carbon Nanospheres
Analytical chemistry (Washington), Vol.82(7), pp.2989-2995
2010
Handle:
https://hdl.handle.net/2376/110869
PMID: 20201502
Abstract
A novel electrochemical immunosensor for sensitive detection of cancer biomarker α-fetoprotein (AFP) is described that uses a graphene sheet sensor platform and functionalized carbon nanospheres (CNSs) labeled with horseradish peroxidase-secondary antibodies (HRP-Ab2). Greatly enhanced sensitivity for the cancer biomarker is based on a dual signal amplification strategy: first, the synthesized CNSs yielded a homogeneous and narrow size distribution, which allowed several binding events of HRP-Ab2 on each nanosphere. Enhanced sensitivity was achieved by introducing the multibioconjugates of HRP-Ab2-CNSs onto the electrode surface through “sandwich” immunoreactions. Second, functionalized graphene sheets used for the biosensor platform increased the surface area to capture a large amount of primary antibodies (Ab1), thus amplifying the detection response. On the basis of the dual signal amplification strategy of graphene sheets and the multienzyme labeling, the developed immunosensor showed a 7-fold increase in detection signal compared to the immunosensor without graphene modification and CNSs labeling. The proposed method could respond to 0.02 ng mL−1 AFP with a linear calibration range from 0.05 to 6 ng mL−1. This amplification strategy is a promising platform for clinical screening of cancer biomarkers and point-of-care diagnostics.
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Details
- Title
- Sensitive Immunosensor for Cancer Biomarker Based on Dual Signal Amplification Strategy of Graphene Sheets and Multienzyme Functionalized Carbon Nanospheres
- Creators
- Dan Du - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaZHEXIANG ZOU - Pacific Northwest National Laboratory, Richland, Washington 99352, United StatesYongsoon SHIN - Pacific Northwest National Laboratory, Richland, Washington 99352, United StatesJUN WANG - Pacific Northwest National Laboratory, Richland, Washington 99352, United StatesHONG WU - Pacific Northwest National Laboratory, Richland, Washington 99352, United StatesMark H ENGELHARD - Pacific Northwest National Laboratory, Richland, Washington 99352, United StatesJun Liu - Pacific Northwest National Laboratory, Richland, Washington 99352, United StatesIlhan A AKSAY - Department of Chemical Engineering, Princeton University, Princeton, New Jersey 08544, United StatesYuehe Lin - Pacific Northwest National Laboratory, Richland, Washington 99352, United States
- Publication Details
- Analytical chemistry (Washington), Vol.82(7), pp.2989-2995
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- American Chemical Society; Washington, DC
- Identifiers
- 99900547344401842
- Language
- English
- Resource Type
- Journal article