Journal article
Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode
Electroanalysis (New York, N.Y.), Vol.20(18), pp.2040-2040
08/05/2008
Handle:
https://hdl.handle.net/2376/116507
PMCID: PMC2817974
PMID: 20148182
Abstract
A rapid, simple and sensitive electrochemical assay of horseradish peroxidase (HRP) performed on disposable screen-printed carbon electrode was developed. HRP activities were monitored by square-wave voltammetric (SWV) measuring the electroactive enzymatic product in the presence of
o
-aminophenol and hydrogen peroxide substrate solution. SWV analysis demonstrated a greater sensitivity and shorter analysis time than the widely used amperometric and differential-pulsed voltammetric methods. The voltammetric characteristics of substrate and enzymatic product as well as the parameters of SWV analysis were optimized. Under optimized conditions, a linear response for HRP from 0.003 - 0.1 U/mL and a detection limit of 0.002 U/mL (1.25×10
−15
mol in 25 μL) were obtained with a good precision (RSD = 8%; n = 6). This rapid and sensitive HRP assay with microliters-assay volume could be readily integrated to portable devices and point-of-care (POC) diagnosis applications.
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Details
- Title
- Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode
- Creators
- Ai-Cheng Lee - Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543Guodong Liu - Pacific Northwest National Laboratory, Richland, Washington 99352Chew-Kiat Heng - Department of Paediatrics, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, SingaporeSwee-Ngin Tan - Academic Group of Natural Sciences and Science Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, SingaporeTit-Meng Lim - Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543Yuehe Lin - Pacific Northwest National Laboratory, Richland, Washington 99352
- Publication Details
- Electroanalysis (New York, N.Y.), Vol.20(18), pp.2040-2040
- Academic Unit
- Mechanical and Materials Engineering, School of
- Identifiers
- 99900547818401842
- Language
- English
- Resource Type
- Journal article