Journal article
Sensitivity of bacteriologic culture for detection of Escherichia coli O157:H7 in bovine feces
Journal of clinical microbiology, Vol.33(10), pp.2616-2619
10/1995
Handle:
https://hdl.handle.net/2376/108239
PMCID: PMC228541
PMID: 8567893
Abstract
The sensitivities of several plating and broth enrichment methods for the detection of Escherichia coli O157:H7 in (i) bovine fecal samples directly inoculated with E. coli O157:H7, (ii) fecal samples from cattle in herds previously positive for E. coli O157:H7, and (iii) fecal samples from calves shedding E. coli O157:H7 after experimental oral inoculation were compared. Three enrichment protocols and three plating protocols were evaluated with directly inoculated fecal samples. All broth enrichment methods were superior to direct plating when they were combined with subsequent plating on sorbitol-MacConkey with cefixime and tellurite (SMACct). SMACct was the most sensitive plating medium, and the three alternative broth enrichment methods gave similar improvements in sensitivity. Of 351 fecal samples from known positive herds, 24 samples (6.8%) were positive by one or more methods. By the most sensitive plating method, cultures of 10-g samples were slightly more sensitive (19 of 351 [5.4%]) than cotton-tipped swab fecal samples (14 of 351 [4.0%]); however, this difference was not significant. For samples from calves orally inoculated with E. coli O157:H7, separation by immunomagnetic beads was slightly more sensitive (79%) than broth enrichment followed by plating at two dilutions (10(-3) and 10(-4)) (71%); however, this difference was not significant. The combination of overnight enrichment of swab fecal samples (0.1 g) and plating on SMACct at two dilutions (10(-3) and 10(-4)) appears to be a sensitive method for detection in large-scale studies involving hundreds of samples per week.
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Details
- Title
- Sensitivity of bacteriologic culture for detection of Escherichia coli O157:H7 in bovine feces
- Creators
- M W Sanderson - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USAJ M Gay - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USAD D Hancock - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USAC C Gay - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USAL K Fox - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USAT E Besser - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USA
- Publication Details
- Journal of clinical microbiology, Vol.33(10), pp.2616-2619
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
- Identifiers
- 99900547744001842
- Language
- English
- Resource Type
- Journal article