Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry

Brian Clowers; Prabha Dwivedi; Wes Steiner; Herbert Hill; Brad Bendiak

doi:10.1016/j.jasms.2005.01.010

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Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry

Journal article

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Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry

Brian Clowers, Prabha Dwivedi, Wes Steiner, Herbert Hill and Brad Bendiak

Journal of the American Society for Mass Spectrometry, Vol.16(5), pp.660-669

05/2005

DOI: https://doi.org/10.1016/j.jasms.2005.01.010

Handle:

https://hdl.handle.net/2376/115465

PMID: 15862767

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Abstract

Biotechnology

Chemistry

Analytical Chemistry

Bioinformatics

Proteomics

Organic Chemistry

A series of isobaric disaccharide-alditols, four derived from O-linked glycoproteins, and select trisaccharides were rapidly resolved using tandem high resolution atmospheric pressure ion-mobility time-of-flight mass spectrometry. Electrospray ionization was used to create the gas-phase sodium adducts of each carbohydrate. Using this technique it was possible to separate up to three isobaric disaccharide alditols and three trisaccharides in the gas phase. Reduced mobility values and experimentally determined ion-neutral cross sections are reported for each sodium-carbohydrate complex. These studies demonstrated that ion mobility separations at atmospheric pressure can provide a high-resolution dimension for analysis of carbohydrate ions that is complementary to traditional mass spectral (m/z) ion analysis. Combining these independent principles for separation of ions provides a powerful new bioanalytical tool for the identification of isomeric carbohydrates.

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Title: Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry
Creators: Brian Clowers - Center for Multiphase Environmental Research Washington State University 99164 Pullman WA USA
Prabha Dwivedi - Department of Chemistry Washington State University 100 Dairy Road 99164 Pullman WA USA
Wes Steiner - Department of Chemistry Washington State University 100 Dairy Road 99164 Pullman WA USA
Herbert Hill - Department of Chemistry Washington State University 100 Dairy Road 99164 Pullman WA USA
Brad Bendiak - Cell and Developmental Biology University of Colorado Health Sciences Center Denver Colorado USA
Publication Details: Journal of the American Society for Mass Spectrometry, Vol.16(5), pp.660-669
Academic Unit: Chemistry, Department of
Publisher: Springer-Verlag; New York
Identifiers: 99900547521501842
Language: English
Resource Type: Journal article